Bacterial-growth-conditions

Bacterial-growth-conditions - Growth & Culture of...

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Growth & Culture of Bacteria
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Chapter 6 Binary Division 1 to 2 to 4 to 8 to ?
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Chapter 6 Phases of Growth Lag Adapt to nutrients Log Active growth Stationary Death = Growth rate Death Nutrients consumed pH too low (why?) Optimize curves in production
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Chapter 6 Log Growth
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Chapter 6
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Chapter 6
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Chapter 6 Measuring Growth • Direct Counts – Petroff-Hauser Chamber – Pro’s vs. Con’s • Serial Dilution – 10-fold serial dilutions – Pro’s vs. Con’s • MPN (Most Probable Number) – Put 10, 1, and 0.1 ml into 10-mls broth • Repeat 5 times per volume – Statistical accurate sampling – Public Health Standards are written for MPN
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Chapter 6
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Chapter 6
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Chapter 6
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Chapter 6
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Chapter 6 Measuring Growth-cont’d • Turbidity – Spectrophotometer – Scale • %Transmittance • Optical Density or Absorbance • Filtration – 0.45 - 0.2 um sizes – Grid Pattern on Filter – Standards for Public Health • 0 E.coli / 100 ml of water – Also used for sterilization
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Chapter 6
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Bacterial-growth-conditions - Growth & Culture of...

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