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Christian12 - as the splice sites and branch point...

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www.usc.edu/si Molecular Biology Supplemental Instruction Christian Kitamura [email protected] SI Worksheet 12 Covers 11/3/08-11/7/08 Topics on this sheet: 1. Poly-A tail addition to pre-mRNA (eukaryotes) 2. Transcription Termination (eukaryotes) 3. Splicing of pre-mRNA with spliceosomes 4. Alternative splicing 5. Initiation of Translation in prokaryotes 1. Name the two proteins that are used to add the poly-adenine tail to pre-mRNA and describe what happens. 2. Complete the pre-mRNA strand with the proper sequence/composition that allows termination of transcription in eukaryotes. Draw in the necessary proteins that carryout the cleavage step in termination. Ds DNA ========================================================== Pre-mRNA 5’-------------------------------------___________------------___________--- 3. What is the consensus sequence for Mammalian introns? Indicate important points such
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Unformatted text preview: as the splice sites and branch point. www.usc.edu/si Molecular Biology Supplemental Instruction Christian Kitamura [email protected] 4. Describe the steps in spliceosome assembly. 5. What are the four types of sequences found in introns & exons? 6. What is the general sequence for an SR protein? What does an SR protein do? 7. Describe the formation of both T antigen and t antigen synthesis in the SV40 virus and show how the splicing affects synthesis. www.usc.edu/si Molecular Biology Supplemental Instruction Christian Kitamura [email protected] 8. How are tRNA’s charged with the proper amino acid? 9. What are the three sites in ribosomes necessary for translation? 10. Describe initiation of translation in prokaryotes and highlight the key charged tRNA required....
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