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Si Review Brandon Wong Week 11

Si Review Brandon Wong Week 11 - Si Review Brandon Wong...

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Si Review Brandon Wong Week 11 Transcription continued… 1.What is the process of termination of eukaryotic transcription? 5’ -pre-mRNA-AAUAA--------G or G/U rich------- 3’ cleavage polyadenylation specificity factor (CPSF) Cleavage Stimulation Factor (CstF) -CFI and CFII -Cuts loose pre-mRNA 2.How is rRNA synthesized? RNA Pol I UBF SLC rRNA B A TBP -150 UCE -100 -1 3.How is tRNA synthesized? RNA Pol III TFIIIB Box A - Box B TBP 4. Besides capping and splicing, what other pre-mRNA modification needs to occur before translation? *poly A Tail Addition of As to 3’ end of pr e-mRNA 1) Poly A polymerase (PAP) + ATP adds only a few As 2) Poly A bind Protein (PAB II) + ATP: adds many more As (50-200 total) -prevents mRNA from being degraded and helps stimulate translation AAAAAAAAA
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RNA Splicing 5. Illustrate the general process of RNA splicing. E1 E2 5’ -----------|*GU ------------------A ------------------*AG|------------ 3’ 5’ss branch point 3’ss *Chambon’s Rule: GU/AG conserved E2 5’ ---------------G--OH- 3’ ----A-----AG|-------- 3’ G 2’ - 5’ bond E1 E2 intron lariat=> degrade by cell 5’ ----------|------------ 3’ + A ------ AG + 5’ -G-G-
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