Problem Set 6 S10 KEY

Problem Set 6 S10 KEY - Problem Set 6 Chapters 19 20...

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Problem Set 6 BILD1 / Spring 2010 Chapters 19 & 20 Dr. Kiger Chapter 19: Viruses 1) A bacterium is infected with an experimentally constructed bacteriophage composed of T2 phage protein coat and T4 phage DNA. The new phages produced would have: (a) T2 protein and T4 DNA (b) T2 protein and T2 DNA (c) a mixture of both T2 and T4 DNA and protein (d) T4 protein and T4 DNA (e) T4 protein and T2 DNA 2) Is a virus alive or dead? Explain your answer. A virus is dead. It is incapable of reproducing outside of a host cell. Viruses lack ATP and are completely inert. Viruses lack the core enzymatic machinery to duplicate genome and copy mRNA. 3) (a) Briefly outline and describe the viral reproductive cycles for: (i) temperate phage: (1) injection of phage DNA into host cell; (2) lysogenic cycle: phage DNA incorporated into host genome (prophage), copied through host DNA replication; (3) environmental signal for lytic cycle: new phage DNA and protein synthesized by host, cells lyse and release amplified phage (ii) virulent phage: (1) injection of phage DNA into host cell; (2) lytic cycle: synthesis of new phage DNA and protein by host cells, assembly of viral particles, cells lyse and release amplified phage (b) What are the names and general mechanisms for the two modes of temperate viral reproduction?
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