CellBio4b - Membranes and the Internal Membrane System...

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Membranes and the Internal Membrane System Chapter 5
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Membrane Transport Mechanisms 1) Passive Diffusion (can transport in both directions) 2) Carrier-Mediated (Facilitated) Diffusion (can transport in both directions) 3) Active (ATP-dependent) Transport is unidirectional Carriers use ATP to pump molecules against a concentration gradient
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Energy-Dependent Channels are Classifed as Uniports Antiports Symports
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Uniports, Symports, Antiports Uniports: transport one substance in one direction Symports: transport two different substances in the same direction Antiports: transport two different substances in opposite directions
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Figure 5.13 Three Types of Proteins for Active Transport
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Additional Important Concepts about Transport Systems Transport can be Primary or Secondary
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With Primary Active Transport- all of the energy comes from Hydrolysis of ATP, even when two molecules are transported
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Figure 5.14 Primary Active Transport: The Sodium–Potassium Pump
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Primary active transport = Sodium-potassium pump
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With Primary Active Transport- all of the energy comes from Hydrolysis of ATP, even when two molecules are transported With Secondary Active Transport, the gradient established by the ATP-dependent primary system powers the transport of the secondary active transport system
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Figure 5.15 Secondary Active Transport
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Watch movie on Active Transport Animation 05-02
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Additional Important Concepts about Transport Systems Transport can be Primary or Secondary Transport Channels can be “gated” either by chemical substances or voltage across the membrane
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Figure 5.10 A Gated Channel Protein Opens in Response to a Stimulus
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5.5. How do Large Molecules Enter and Leave a Cell?
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Process by which complex molecules, bacterial cells, and water can enter cells is called Endocytosis Moving Molecules/Particles Into and Out of Cells
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Figure 5.16 Endocytosis and Exocytosis (A)
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Endocytosis A membrane vesicle -part of the plasma membrane -forms around a particle or a bacterium Membrane vesicle is brought into the cell
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Endocytosis 1. Phagocytosis : large particles and small cells are engulfed inside vesicles Three kinds:
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Phagocytosis of Bacteria by a Macrophage
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Phagocytosis movie http://mcbi.ouhsc.edu/clarkelab/phagocytosis_movies/Clarke_Movie1 .mov Dictyostelium amoebae, strain AX2 are stained in red. The bacteria ( E. coli B/r) express GFP (green), and the amoebae are expressing fluorescent labels for actin or microtubules.
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Endocytosis 1. Phagocytosis: large particles and small cells are engulfed inside vesicles 2. Pinocytosis (or ”cell drinking”): cells take up liquid into vesicles Three kinds:
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Endocytosis 1. Phagocytosis: large particles and small cells are engulfed inside vesicles 2. Pinocytosis (or ”cell drinking”): cells take up water
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CellBio4b - Membranes and the Internal Membrane System...

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