MCDB_1AL_Lab_Quiz_2_

MCDB_1AL_Lab_Quiz_2_ - Name _ TA _ MCDB 1AL Lab Quiz 2 Nov....

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Name _________________________ TA ________________________ MCDB 1AL Lab Quiz 2 Nov. 17-21, 2008 Scantron Instructions: 1. Use a #2 pencil to complete the form. 2. Write your name and fill in the appropriate bubbles. 3. Write your perm. number in the ID number box and fill in the bubbles. 4. Fill in the entire rectangle of the answer you choose. If you erase, erase completely READ ALL QUESTIONS THOROUGHLY----IN ALL CASES, PICK THE BEST ANSWER 25 QUESTIONS - 1 POINT EACH 1. These tubes are the result of a Bradford Protein assay performed just as you did in lab (recall that Bradford protein is measured at 595 nm). Which tube has the highest concentration of protein? A. Tube 1 has the highest protein concentration B. Tube 2 has the highest protein concentration C. Impossible to tell without a spectrophotometer 2. Your lab partner has set up a an agarose gel to determine the size of products from a PCR reaction. Before she starts the gel, you look at it and have the following comments: A. This is set up perfectly, thanks very much! B. The polarity of the gel is reversed and we will lose our DNA. C. The buffer level in the tank is too low so our electrophoresis won't run. D. The buffer level in the tank is too high, our samples will wash away. E. You should not have added the blue tracking dye, it will interfere with our ability to see the DNA. 3. If this pipetman is used to transfer a volume of water from one beaker to another TWICE, how much water is transferred? A. 50 ul B. 125 ul C. 150 ul D. all of the above E. none of the above 4. Ethidium bromide is used in Agarose Electrophoresis of DNA because. ..... A. It allows the progress of electrophoresis to be monitored B. It allows DNA to be visualized because it fluoresces when bound to DNA C. It neutralizes the charge on DNA and thereby allows DNA to move evenly through the gel D. It appears blue to the naked eye E. More than one of the above is correct MCDB 1AL Lab Quiz 2 Version: 0 Page: 1
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5. In order to determine the concentration of BSA in a sample, we measured the peak absorbance at 595 nm. What, specifically, is absorbing maximally at 595 nm?
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This note was uploaded on 10/16/2010 for the course MCDB MCDB 1A taught by Professor Senghuilow during the Spring '09 term at UCSB.

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MCDB_1AL_Lab_Quiz_2_ - Name _ TA _ MCDB 1AL Lab Quiz 2 Nov....

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