MCDB_1AL_Quiz_2_Summer_2009

MCDB_1AL_Quiz_2_Summer_2009 - NAME_ TA_ MCDB 1AL SUMMER...

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NAME______________________ TA______________________ MCDB 1AL SUMMER 2009 QUIZ 2 INSTRUCTIONS: 1. Use a #2 pencil for the scantron 2. Put your name on both the scantron and exam 3. Fill in the entire rectangle on the scantron. If you erase, erase completely. 4. Read all questions thoroughly. In all cases, pick the best answer. There are a total of 35 questions. The first 5 are practicals. 1. In the small vial is a red protein dissolved in nanopure water. Prepare a diluted solution by combining 3mL of water and 150uL of the red protein solution. Use the spectrophotometer to measure the absorbance of your solution at 550nm. The absorbance of your solution is (choose the closest value): a. 0.030 b. 0.260 c. 0.310 d. 0.660 e. 2.600 Before you leave this question: i. Remove all tubes from the spec ii. Press 0% A/T iii. Set any pipets you used to zero iv. Clean up (pour liquids out of tubes that you made, throw tips and tubes in waste, slide 5mL pipet back into sleeve) 2. The sample on this slide is: (PLEASE REMOVE THE SLIDE AND RETURN THE MICROSCOPE TO THE “END OF USE” POSITION WHEN YOU LEAVE THIS QUESTION.) a. Sea star (starfish) embryo b. Whitefish blastula c. Root tip d. Squash of Drosophila salivary gland e. Urchin sperm 3. If you were to pipet once from each of these pipettors, what would be the total volume dispensed? a. 51.9 uL b. 69.0 uL c. 321.9 uL d. 339.0 uL e. 519.0 uL
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4. Which of the following is NOT true for this solution? a. It will show some absorbance of green light b. It will show high transmittance for light at 595 nm c. It will show some absorbance of orange light d. It reflects violet light 5. Your lab partner has set up an agarose gel to determine the size of products from a PCR reaction. Before she starts the gel, you look at it and have the following comment: (Please select the appropriate response to the set up seen here) a. This is set up perfectly, thank you very much! b. The buffer level in the tank is too low so our electrophoresis won’t run. c. The polarity of the gel is reversed and we will lose our DNA. d. The buffer level in the tank is too high, our samples will wash away. 6. Which of the following nuclei can undergo mitosis? a. Haploid b. Diploid c. Polyploid d. All of the above e. B and c only 7. A blastula is: a. a hollow sphere of cells undergoing rapid mitotic division shortly after fertilization b. the diploid cell that forms as a result of fertilization c. a ball of cells that forms shortly after fertilization by meiotic divisions d. the lesser known brother of Dracula e. one of the cells of a young embryo 8. Four tubes are sequentially labeled A through D. Tube A has 2 ml of a 100mg/ml BSA solution and tubes B, C, and D each have 1 ml of water. If you take 1 ml from tube A and put it into tube B, then take one ml of the resulting solution and put it into tube C, then take one ml of the resulting solution and put it into tube D, what is the final concentration of BSA in tube D? a.
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MCDB_1AL_Quiz_2_Summer_2009 - NAME_ TA_ MCDB 1AL SUMMER...

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