MCDB_1AL_Quiz_3_Summer_2009

MCDB_1AL_Quiz_3_Summer_2009 - NAME TA MCDB 1AL SUMMER 2009...

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NAME______________________ TA______________________ MCDB 1AL SUMMER 2009 QUIZ 3 INSTRUCTIONS: 1. Use a #2 pencil for the scantron 2. Put your name on both the scantron and exam 3. Fill in the entire rectangle on the scantron. If you erase, erase completely. 4. Read all questions thoroughly. In all cases, pick the best answer. There are a total of 36 questions. The first 4 are practicals. 1. Using only one pipet here and dispensing only once, what is the weight of 0.600 mL of this solution? Please throw away your weigh boat and set pipets back to zero when you are finished. a. 0.680 mg b. 680 mg c. 600 g d. 680 g e. 600 mg 2. If you were to pipet once from each of these pipettors, what would be the total volume dispensed? a. 122.1 uL b. 321.0 uL c. 1022.1 uL d. 1202.1 uL e. 1221.0 uL 3. TPA 25 was amplified from cheek-cell DNA by PCR and the products run on an agarose gel just as you did in lab. In the gel shown here, samples were loaded onto the gel in the following order (from left to right): Lane 1: pGEM standards Lane 2: PCR product of individual A Lane 3: PCR product of individual B Lane 4: PCR product of individual C For which individual(s) can you definitively (i.e. unambiguously) say that TPA25 with the ALU insert is NOT present in the entire genome? a. Individual A (lane 2) b. Individual B (lane 3) c. Individual C (lane 4) d. Both Individual A (lane 2) and B (lane 3) e. Both Individual B (lane 3) and C (lane 4)
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4. Recall that in the Bradford protein assay, protein concentration is proportional to absorbance at 595 nm. The Bradford assay has been performed on these two solutions (A and B) just as you did in your laboratory. Which of them, A or B, has the highest concentration of protein? a. Solution A b. Solution B c. It is impossible to tell without a standard curve. d. It is impossible to tell without measuring absorbance. e. Both c and d 5. For the image of the urchin embryo below, the tissue denoted by the arrow will become the: a. Archenteron b. Mesoderm c. Ectoderm d. Endoderm e. Blastula For the next 2 questions use the following information: A frog cell in interphase, before S phase of the cell cycle, has 20 pg (i.e. 20*10 -12 grams) of DNA distributed among 20 chromosomes. 6. At metaphase of mitosis, each cell contains _______ separate chromosomes. a. 10 b. 20 c. 40 d. 80 e. none of the above 7. At metaphase of mitosis, each cell contains _______ pg of DNA. a. 10 b. 20 c. 40 d. 80 e. none of the above
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8. Given the following dilution scheme (described and diagrammed below), what was the original concentration of protein in tube “X” if the final concentration in tube 3 is 10 μ g/ml: i. Tube “X” = unknown original concentration ii. Tube 1: 1 mL of tube “X” was mixed with 3 mL water iii. Tube 2: 2 mL of tube 1 was mixed with 3 mL of water iv. Tube 3: 1 mL of tube 2 was mixed with 9 mL of water a. 20 μ g/mL b. 100 μ g/mL c. 250 μ g/mL d. 810 μ g/mL e. 1 mg/mL 9. The diagram below best describes how enzymes: a. increase the rate of a chemical reaction. b.
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This note was uploaded on 10/16/2010 for the course MCDB MCDB 1A taught by Professor Senghuilow during the Spring '09 term at UCSB.

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MCDB_1AL_Quiz_3_Summer_2009 - NAME TA MCDB 1AL SUMMER 2009...

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