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Unformatted text preview: medium longer, so they wont travel as far. Molecules are retained more or less based on their polarity; the most non-polar species go the farthest and the most polar go the least distance. Thus, when we repeat the experiment at the same condition, the R f value for a same ingredient should be exactly the same. However, I think the Thin-layer Chromatography has it own limitations. For instance, if the concentration of a ingredient in the tablet is too low, the dot wont show in the plane....
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- Spring '09