Analytical answers chp10

Analytical answers chp10 - Analytical Questions...

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Analytical Questions 1. Transcription of the class II gene start at a guanosine 30 bp downstream of the last base of TATA box. You delete 20 bp of DNA between this guanosine and the TATA box and transfect cells w/this mutated DNA. Will transcription still start at the same guanosine? If not, where? How would you locate the same transcription start site? Ans: No, transcription will not start in the same place. It will start about 20 bp downstream of its normal start site. You can locate the new start site with either S1 mapping or primer extension analysis. The latter method will give better resolution. 2. You suspect that a repeated sequence just upstream of a gene is acting as an enhancer. Describe and predict the results of an experiment you would run to test your hypothesis. Be sure your experiment shows that the sequence acts as an enhancer and not as a promoter element. Ans: ***ENHANCES STIMULATES TRANSCRIPTION (ARE ORIENTATION AND POSITION INDEPENDENT DNA ELEMENTS) *SILENCES TURN OFF TRANSCRIPTION
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Analytical answers chp10 - Analytical Questions...

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