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Unformatted text preview: The foreign DNA is cleaved with the same restriction enzyme, giving another rod with complementary, sticky ends. By chance, some plasmids will circularize into plasmids once some, some of which will contain insertion of the foreign DNA. Bacterial cells are then transfected by the plasmids, and the colonies grown. Genes for two antibiotic resistance conferring genes are used, because the plasmid is typically opened with one resistance-conferring gene. If foreign DNA is inserted into that gene, progeny colonies will lose their resistance to that antibiotic. The other gene is used to determine whether bacteria carry the plasmids at all, whether or not they contain DNA for the foreign gene. Use of the two markers will allow colonies to be selected that carry the recombinant plasmid. 30. Omitted 31. Omitted...
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This note was uploaded on 10/20/2010 for the course MCB 01 taught by Professor Mintel during the Summer '09 term at University of Illinois, Urbana Champaign.
- Summer '09