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ex 1 key - The foreign DNA is cleaved with the same...

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MCB 450 Examination 1 – Key Spring 2010 1. E 2. E 3. C 4. D 5. A 6. B 7. A 8. C 9. E 10. C 11. E 12. D 13. C 14. E 15. B 14. E 15. B 16. E 17. C 18. B 19. A 20. C 21. Either 22. 15.8 23. a) -9.5 kJ/mol, b) Cells might not have an enzyme catalyzing the reaction. 24. -0.23 kJ/mol (Need to express R in kJ. Must express conc in M.) 25. a) 1; b) α carboxyl 26. Omitted 27. Water can no longer form an iceberg-like cage around the hydrocarbon tail. Rather the water is randomized. The driving force for hydrophobic bond formation is entropic. 28. Near the exterior surfact. H-bonds are weak, polar interactions between a partially positively charged peptide hydrogen, and a partially negatively charged carbonyl oxygen. The strength of polar interactions is inversely proportional to the dielectric constant, D, which is much lower on the interior of a globular protein. 29. A plasmid is opened by a restriction endonuclease into a rod with sticky ends.
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Unformatted text preview: The foreign DNA is cleaved with the same restriction enzyme, giving another rod with complementary, sticky ends. By chance, some plasmids will circularize into plasmids once some, some of which will contain insertion of the foreign DNA. Bacterial cells are then transfected by the plasmids, and the colonies grown. Genes for two antibiotic resistance conferring genes are used, because the plasmid is typically opened with one resistance-conferring gene. If foreign DNA is inserted into that gene, progeny colonies will lose their resistance to that antibiotic. The other gene is used to determine whether bacteria carry the plasmids at all, whether or not they contain DNA for the foreign gene. Use of the two markers will allow colonies to be selected that carry the recombinant plasmid. 30. Omitted 31. Omitted...
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