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problem_set_1_sp09-1

problem_set_1_sp09-1 - Ala Arg Gly Met Ser Thr Tyr Val a...

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MCB 450 Problem Set 1 1. If the internal pH of a muscle cell is 6.8, what is the [HPO 4 2- ]/[H 2 PO 4 - ] ratio? (pK 2 = 7.2) 2. What is the [H 2 PO 4 - ]/H 3 PO 4 ratio at pH 3.0? At pH 2.0? (pK 1 = 2.15) 3. The free energies of hydrolysis for several compounds are given in the table below: Compound G o′ (kJ/mol) Creatine phosphate -43.3 ATP (to ADP + phosphate) -30.5 Glucose 6-phosphate -13.9 Glucose 1-phosphate -21.0 Calculate free energy changes for the following reactions. a) Creatine phosphate + ADP = creatine + ATP b) Glucose + ATP = glucose 6-phosphate + ADP c) Glucose 6-phosphate = glucose 1-phosphate 4. Calculate the free energy of hydrolysis of ATP in a rat liver cell where the concentrations of ATP, ADP, and Pi are 3.4, 1.3, 4.8 mM respectively. Assume a temperature of 37 C. 5.

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Unformatted text preview: Ala, Arg, Gly, Met, Ser, Thr, Tyr, Val a) Treatment of the original peptide with dansyl chloride followed by acid hydrolysis yielded a dansyl-valine derivative. b) Treatment of the original peptide with trypsin yielded two tetrapeptides. c) Treatment of the original peptide with chymotrypsin yielded free Gly and another product. d) Treatment of the original peptide with cyanogen bromide yielded a dipeptide containing Gly and another product. e) Partial acid hydrolysis of the original octapeptide yielded the dipeptide Thr-Ala, among other products. Deduce a sequence for the octapeptide that is consistent with the foregoing information....
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