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ProblemSet2_SP09 - 4 If glyceraldehyde labeled with 14 C in...

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MCB 450 Problem Set 2 1. From the following data on an enzymic reaction, determine (a) the type of inhibition, (b) K M for the substrate, (c) V max , and (d) K i , assuming that the inhibitor is present at a final concentration of 5mM in the reaction mixture. Substrate Concentration (mM) Product per hour ( μ g) No Inhibitor With Inhibitor 2.0 139 88 3.0 179 121 4.0 213 149 10.0 313 257 15.0 370 313 2. Below are shown two dissociation curves for hemoglobin. Decide which curve corresponds to: _____ the higher pH _____ the higher partial pressure of carbon dioxide _____ the higher concentration of 2,3 BPG %Sat 1 2 pO 2
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3. For each of the substrates and products given below, decide what would be the net gain in ATP as one molecule of substrate is converted to product. Substrate Product # ATP Fructose Lactate Galactose Pyruvate Glyceraldehyde Lactate (Add parts on complete combustion.)
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Unformatted text preview: 4. If glyceraldehyde labeled with 14 C in its aldehyde carbon atom is added to a glycolytic preparation, which carbon atom of pyruvate would be expected to be labeled? 5. For each turn of the citric acid cycle, how many of each of the following is(are) produced? _____ NADH _____ GTP _____ CO 2 _____ FADH 2 6. When succinate labeled in one of its carboxyl groups is added to isolated mitochondria, oxaloacetate labeled in both of its carboxyl groups can be subsequently isolated. Propose an explanation for this observation. 7. The standard reduction potential for (NAD + /NADH) is -0.32 volts, and that for (pyruvate, lactate) is -0.185 volts. (a) Calculate Δ E o ′ for the following reaction: pyruvate + NADH + H + = lactate + NAD + (b) Calculate Δ G o ′ for the above reaction. (F = 96,485 J · V-1 · mol-1 )...
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