BIS101 HW6 F08 Engebrecht

BIS101 HW6 F08 Engebrecht - Deletions x Point mutations 1 2...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIS101/Engebrecht Homework06 Chapter 10 in 7 th edition: 5, 11, 18 Chapter 9 in 8 th edition: 6, 25, 29 Chapter 9 in 9 th edition: 6, 28, 32 1. Five single rIIA deletion mutants of phage T4 were co-infected into E. coli B in all pairwise combinations to test for their ability to give rII+ recombinants on E. coli K. The results are given in the table to the left below. Five different point mutations were then tested in the same way for their ability to recombine with the five deletions. These results are given in the table to the right below. + = recombinant wild-type phage are detected; - = no recombinant wild-type phage are detected. Please draw a linear map representing the order and overlap of the deletions and the positions of the point mutations. Deletions x Deletions
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Deletions x Point mutations 1 2 3 4 5 1 2 3 4 5 1 - - - - + a + - - + + 2 - - + + b + + - + - 3 - - - c - + - - + 4 - + d - - - + + 5 - e + + - - + 2. The following DNA is part of a gene that codes for a polypeptide of at least seven amino acids: 3' c a a t t g a t t a g t c a g t c a a t t g a t 5' 5' g t t a a c t a a t c a g t c a g t t a a c t a 3' Please see codon table on next page to answer the questions below: a) Which strand includes an Open Reading Frame? Top (T) or Bottom (B) b) Which DNA strand is the sense strand? Top (T) or Bottom (B) c) Give the mRNA sequence that would be transcribed from this gene; label the 5' & 3' ends. d) Give the seven amino acids that would be translated from the correct message; label the N & C ends....
View Full Document

{[ snackBarMessage ]}

Page1 / 2

BIS101 HW6 F08 Engebrecht - Deletions x Point mutations 1 2...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online