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BIS101 HW6 F08 Key Engebrecht

BIS101 HW6 F08 Key Engebrecht - 1 2 4 5 a d c e b OR 3 1 5...

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BIS101/Engebrecht Homework06 1. Five single rIIA deletion mutants of phage T4 were co-infected into E. coli B in all pairwise combinations to test for their ability to give rII+ recombinants on E. coli K. The results are given in the table to the left below. Five different point mutations were then tested in the same way for their ability to recombine with the five deletions. These results are given in the table to the right below. + = recombinant wild-type phage are detected; - = no recombinant wild-type phage are detected. Please draw a linear map representing the order and overlap of the deletions and the positions of the point mutations. Deletions x Deletions Deletions x Point mutations 1 2 3 4 5 1 2 3 4 5 1 - - - - + a + - - + + 2 - - + + b + + - + - 3 - - - c - + - - + 4 - + d - - - + + 5 - e + + - - + 3_________________________
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Unformatted text preview: 1________ 2_______ 4_____ 5____ a d c e b OR 3_________________________ 1________ 5____ 2_______ 4_____ b a d c e 2. The following DNA is part of a gene that codes for a polypeptide of at least seven amino acids: 3' c a a t t g a t t a g t c a g t c a a t t g a t 5' 5' g t t a a c t a a t c a g t c a g t t a a c t a 3' Please see codon table on next page to answer the questions below: a) Which strand includes an Open Reading Frame? Top (T) or Bottom (B) B b) Which DNA strand is the sense strand? Top (T) or Bottom (B) B c) Give the mRNA sequence that would be transcribed from this gene; label the 5' & 3' ends. 5’ g u u a a c u a a u c a g u c a g u u a a c u a 3’ d) Give the seven amino acids that would be translated from the correct message; label the C & N ends. N- Leu – Thr – Asn – Gln – Ser – Val – Asn – C...
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