BIS101 HW8 F08 Key Engebrecht

BIS101 HW8 F08 Key Engebrecht - BIS101/Engebrecht Homework...

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Homework #8 In 7 th edition In 8 th edition Chapter 12: Chapter 11: 1, 7, 9, 10, 12, 17, 20a-c 17, 21, 22, 29, 31a-c Chapter 13: Chapter 11: 5, 8 34, 35 Chapter 14: Chapter 12: 3, 11, 12 32, 35, 36 1. You have isolated seven yeast mutants that fail to grow in the absence of histidine. You perform complementation tests and obtain the following results: (Remember numbers indicate mutant and + means wild type while – indicates that the cross generated a mutant phenotype) 1 2 3 4 5 6 7 1 - + + + - - + 2 - + + + + - 3 - + + + + 4 - + + + 5 - - + 6 - + 7 - a. How many complementation groups are there and which mutants fall into each group? 4 groups; mutants: 1,5,6; 2,7; 3; 4 b. (10pts) You are particularly interested in mutant 4, which you name his4 and find that it is linked to leu2 and fus1 on chromosome III. You perform a 3-point cross of the type HIS4 LEU2 FUS1 x his4 leu2 fus1 and obtained the results shown below. Capital letters denote wild-type genes, lower case letters denote mutant genes. Based on these results, what is the gene order and what is the genetic distance between the genes? Draw a diagram giving the order and the
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This note was uploaded on 10/22/2010 for the course BIS 101 taught by Professor Simonchan during the Fall '08 term at UC Davis.

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BIS101 HW8 F08 Key Engebrecht - BIS101/Engebrecht Homework...

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