BIS101 MT2 F08 Key Engebrecht

BIS101 MT2 F08 Key Engebrecht - BIS 101-003 (Engebrecht)...

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BIS 101-003 (Engebrecht) Name: ______________________________ Fall 2008 Last, First ID #:_______________________________ BIS 101-003 - MIDTERM IIAKey This exam has a total of 100 points. READ THE QUESTIONS CAREFULLY BEFORE YOU ANSWER. SHOW ALL WORK TO GET FULL CREDIT. Please sign your name on the score sheet. This exam has seven pages including this cover page and the score sheet HONOR CODE: My signature below affirms that I wrote this exam in the spirit of the honor system of the University of California, Davis. I neither received nor furnished any “help” during the exam, nor did I use any unauthorized references. Signature: ______________________________________________________ Good luck Authorization for Public Distribution of Graded Examination I, ______________________________________, ID# _______________________ authorize that after this exam is graded, it can be placed in a holding bin, labeled BIS 101- 003/Engebrecht, on the first floor of Briggs Hall.
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1. (6pts) In a generalized transduction experiment donor E. coli have the genotype his+ trp+ gal- and recipient cells have the genotype his- trp- gal+. You perform the following two experiments: Experiment Selected marker Unselected markers (co-transduction frequency) 1 his 10% trp+, 30% gal- 2 trp 50% gal-, 10% his+ a) (3pts) Which genes are the closest to each other? Why? Trp and gal since they have the highest co-transduction frequency b) 34pts)What is the order of the three genes? his gal trp or trp gal his 2. (10pts) You extract total genomic DNA from E. coli , C. elegans and mouse. You perform a denaturation experiment and see the following: a) (3 pts) What does this tell you about these three genomes? C. elegans has the lowest GC content, mouse is intermediate and E. coli has the highest GC content . b) (3pts) If C. elegans has 32% A, please indicate the % composition of each of the remaining bases: G = 18 C = 18 T = 32 C. elegans Mouse E. coli
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c) (4pts) You perform renaturation experiments with E. coli and mouse DNA and see the the following: (2pts) Indicate which curves correspond to E. coli and mouse by labeling the graphs above. (2pts) What do these renaturation curves suggest about these genomes?
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BIS101 MT2 F08 Key Engebrecht - BIS 101-003 (Engebrecht)...

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