BIS101 MT2 S07 Key Engebrecht

BIS101 MT2 S07 Key Engebrecht - BIS 101-02 (Engebrecht)...

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BIS 101-02 (Engebrecht) Name: ______________________________ Spring 2007 Last, First ID #:_______________________________ BIS 101-02 - MIDTERM IIAKey This exam has a total of 100 points. READ THE QUESTIONS CAREFULLY BEFORE YOU ANSWER. SHOW ALL WORK TO GET FULL CREDIT. Please sign your name on the score sheet. This exam has seven pages including this cover page and the score sheet HONOR CODE: My signature below affirms that I wrote this exam in the spirit of the honor system of the University of California, Davis. I neither received nor furnished any “help” during the exam, nor did I use any unauthorized references. Signature: ______________________________________________________ Good luck Authorization for Public Distribution of Graded Examination I, ______________________________________, ID# _______________________ authorize that after this exam is graded, it can be placed in a holding bin, labeled BIS 101- 02/Engebrecht, on the first floor of Briggs Hall.
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1. (5pts) In a generalized transduction experiment donor E. coli have the genotype his+ trp+ gal- and recipient cells have the genotype his- trp- gal+. P1 mediated transductants for his+ were selected, and their total genotypes were determined, with the following results: Genotype Number of progeny his+ trp+ gal- 205 his+ trp- gal- 95 his+ trp+ gal+ 5 his+ trp- gal+ 195 500 What is the order of the three genes? The donor is his+ trp+ gal-, the recipient is his- trp- gal-, and the rare class is his+ trp+ gal+. Since gal is not transferred but the other two are, gal must be in the middle. The order is: his gal trp or trp gal his. 2. (10pts) A friend has asked you to characterize the genomes of three recently isolated viruses, named Davirus1, Davirus2, and Davirus3. She gives you a sample of each genome at a concentration of 10 μ g/ml. You perform a denaturation experiment and see the following: a) (3 pts) Indicate if the viral genomes are single stranded or double stranded: Davirus1 single stranded Davirus2 double stranded Davirus3 double stranded b) (2pts) What does the denaturation kinetics tells you about the Davirus2 and Davirus3 genomes? Davirus3 has a higher GC content than Davirus2.
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c) (3pts) Analysis of base composition reveals the following Davirus1: 30% guanine; 30% adenine; 20% cytosine; 20% uracil Davirus2: 20% guanine; 30% adenine; 20% cytosine; 30% thymine Davirus3: 30% guanine; 20% adenine; 30% cytosine; 20% thymine Indicate the type of nucleic acid each viral genome is made of. Davirus1: RNA Davirus2: DNA Davirus3: DNA d) (2pts) Renaturation kinetics of Davirus2 and Davirus3 show the following: Which virus has a larger genome? Davirus2 must have a larger genome because it took longer to reanneal suggesting
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BIS101 MT2 S07 Key Engebrecht - BIS 101-02 (Engebrecht)...

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