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Unformatted text preview: following diene (4 pts). Br 2 Br Br 3. Name the following compounds (4 pts total, 2pts each). Cl Br O 2 N OH 4-bromo-3-chlorotoluene 3-nitrophenol 4. The weight of proteins or nucleic acids in solution is commonly determined by UV spectroscopy using the Beer-Lambert law. For example, the ε of double-stranded DNA at 260 nm is 6670 M-1 cm-1 . The formula weight of the repeating unit in DNA (650 grams/mol) can be used as the molecular weight. What is the amount (mg) of DNA in 2.0 ml of aqueous buffer if the absorbance, measured in a 1 cm cuvette, is 0.75? (5 pts) A= ε Cl C=0.75/(6670*1) C=1.124*10-4 M Amount of DNA=1.124*10-4 *650*(2*10-3 ) =0.146mg Page 2 of 2...
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This note was uploaded on 10/22/2010 for the course CHE 128C taught by Professor Chen during the Spring '07 term at UC Davis.
- Spring '07
- Organic chemistry