BIS103 MT2 S09 Callis

BIS103 MT2 S09 Callis - BIS103-001 MIDTERM 2 Spring 09...

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BIS103-001 MIDTERM 2 Spring 09 Instructor: Callis Name__________________________________________________ May 21, 2009 LAST NAME, FIRST NAME Please print clearly 1 Check all the appropriate box or boxes below that apply to you: Completing Incomplete Senior Graduating Spring 09 Aggie Fan Note: There are 6 questions and a total of 14 numbered pages. Partial credit will be given. WRITE YOUR NAME ON THE TOP OF EACH PAGE, last name first. A blank sheet will be provided as scratch paper and to cover your exam. T= 298º K R= 8.314 J x mol -1 x K -1 Faraday's constant (F) is 96.5kJ x volt -1 x mol -1 Δ G o ' for the reaction ADP + P i ATP + H 2 O = + 30.5 kJ x mole -1 Δ G o ' = - n F Δ E o ' Δ G = Δ G o ' + RT ln [products/reactants] Δ E o ' = E o ' (a) - E o ' (d) for Aox + 1 e- Ared E = E o ' + RT ln A ox nF A red I, ______________________________, authorize the University to distribute publicly this graded exam ( e.g. , handed out in class, or left in a bin for me to pick up). Signature ________________________________________ Date _____________________ Honor Code: My signature below affirms that I wrote this exam in the spirit of the honor system of UCD. I neither received nor furnished any help during the exam. Signature ________________________________________
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BIS103-001 MIDTERM 2 Spring 09 Instructor: Callis Name__________________________________________________ May 21, 2009 LAST NAME, FIRST NAME Please print clearly 2 Student ID # _______________________________________ Question Value Score 1 12 2 22 3 14 4 18 5 20 6 14 TOTAL 100
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BIS103-001 MIDTERM 2 Spring 09 Instructor: Callis Name__________________________________________________ May 21, 2009 LAST NAME, FIRST NAME Please print clearly 3 1. (12 pts). 2,4-Dinitrophenol (2,4-DNP) has a profound effect on oxidative phosphorylation and electron transport. 2,4-DNP is soluble in membranes and in water and can easily move across membranes. It also is a weak acid that picks up and releases protons (H + ) easily. It is these two properties that are thought to lead to its effect on oxidative phosphorylation and electron transport. Please explain the following phenomena on the basis of our current understanding of energy generation in mitochondria. Assume all compounds can enter the mitochondrial matrix. (a). (4 pts). To a test tube of isolated human mitochondria suspended in a buffer and osmoticum, NADH, 2,4-DNP, ADP, Pi and oxygen are added. Oxygen is consumed, but ATP is not synthesized. Briefly explain why. (b). (4 pts). To a test tube of isolated human mitochondria suspended in a buffer and osmoticum, NADH, Pi and oxygen are added, but not ADP. There is no oxygen consumption until 2,4-DNP is added. Briefly explain why.
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BIS103-001 MIDTERM 2 Spring 09 Instructor: Callis Name__________________________________________________ May 21, 2009 LAST NAME, FIRST NAME Please print clearly 4 (c). (4 pts). Accidental consumption of 2,4-DNP by humans leads to an increase in body temperature and profuse sweating. Based on your knowledge of 2,4-DNP explain the basis for this effect. 2. (22 pts). (a). 6 pts. Some bacterial species accumulate beta-hydroxybutryic acid as a form of stored energy. How many ATPs are produced when one mole of this molecule is completely oxidized to CO 2 + H 2 O via beta oxidation and the TCA cycle and the reduced electron carriers are oxidized in the electron transport chain? For complete credit, show calculations and sources of ATP. Write your final answer on the line provided.
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