BIS103 MT2 S10 KEY Callis

BIS103 MT2 S10 KEY - 318103-001 — MIDTERM 2 Name k 3 Last First(print clearly please 1(10 pts Fill in the blanks by writing the answer on the

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Unformatted text preview: 318103-001 — MIDTERM 2 May 20, 2010 Name k 3 Last, First (print clearly, please) 1. (10 pts). Fill in the blanks by writing the answer on the line below with the same number. Each answer is worth 1 pt. The answer could be a word, a structure, a phrase or a sentence. The condition called ketosis that occurs in humans results from the production of a (name a class of compounds). The name g structure of one of these compounds named in (a) is b . The source of these compounds is 0 (name a storage material). Ketosis occurs because d (name a metabolite) is not present at sufficient concentrations, nor synthesized at a sufficient rate. To reduce ketosis, a person should e a. EETDNi g oQIiS a “ ‘ ‘”‘ 0H maybe»; CH3 C, CHLQoa I p I“- i - b. METDNZ or magma av (5-0.4 gummy-g c413 (fiat/law —_—_— H c. TKmMLeL‘lCieLDZ d. 0AA (TCA can: meanime e. EAT SMGAL§ em mmwvme) The Gibbs free energy released in the reactions catalyzed by the complexes of the Electron Transport Chain is used directly to f . The initial electron donors into the electron transport chain are g and h . The terminal electron acceptor is i , converting it to j . f. Maui Paofowg AbAlU‘JT Lon/cm rKArzoN rue/1019M . MDT NC WDCCE GIG/Hi. Paton) LPN) 318103-001 — MIDTERM 2 K E 7 May 20, 2010 Name 4 Last, First (print clearly, please) 2. (10 pts). Based on your knewledge of the roles of adrenaline, insulin and glucagon in regulating fuel metabolism in humans, for each hormonal change, predict the effect that the hormone has on the flux of a metabolic pathway, concentration of a compound, or enzyme activity listed below each hormonal change. Circle either INCREASE, DECREASE, or NO CHANGE. CIRCLE ONE for each row Release of adrenaline from adrenal medulla CAMP levels in muscle DECREASE NO CHANGE glycogen synthesis in muscle INCREASE NO CHANGE Release of insulin from pancreatic beta cells: CAMP levels in adipose cells INCREASE NO CHANGE glycogen synthesis in liver DECREASE NO CHANGE protein synthesis DECREASE NO CHANGE glucose uptake into liver cells from blood " DECREASE NO CHANGE Release of glucagon from pancreatic alpha cells: CAMP levels in liver INCREASE DECREASE NO CHANGE phosphorylase activity in muscle INCREASE DECREASE glycogen synthesis in liver INCREASE CREA NO CHANGE glycogen breakdown in liver DECREASE NO CHANGE 315103-001 — MIDTERM 2 E May 20, 2010 Name 5 Last, First (print clearly, please) 3. Enzyme Regulation (18 pts) a. (4 pts) What is futile cycling (also known as substrate cycling)? Define this term. Explain the difference between complete and partial futile cycling. Be briefl @peosmod ‘1an a+ «Mr Sam Wq Y “WW W W 1s WM memoir SMWJJJ, 3:0 w) MT LD’hMW 0W3. PMEJZ W \5 MW Vales n5!” MEN/J.) 30% Ni WWW W6 . b. (3 pts) Briefly describe one metabolic advantage to partial futile cycling. lac/(ems! tort» "gfiwzwtn 2? W h M 49%, lawman, MW.4L+DMM.+~W My. Mata NAM‘MWW atom \ \ 0’),ch l Mal/1mv Wish/v4 W W4» 01¢. c. (3 pts) Briefly describe one metabolic disadvantage to partial futile cycling. MM (5 WWW Baa/£1, r49 e,th wMaQ , um msfi MP “A NAM. W «awammu my? hr WWW QW‘ 315103-001 — MIDTERM 2 K 5 May 20, 2010 Name 6 Last, First (print clearly, please) (1). (8 pts). Below are the names of several enzymes that are allosterically regulated. Their corresponding allosteric effectors are listed below each enzyme. Predict the effect that binding of each allosteric effector would have on the specific activity of the enzyme towards its substrate(s). WRITE "A" for activating or an “I” for inhibiting on the line provided. i). Phosphofructokinase-l (PFK- 1) ATP 3: A F2,6 bisP ii). Acetyl CoA carboxylase acetyl CoA A citrate A iii). Bacterial isocitrate lyase ADP I— iv) glycogen synthase glucose-6-P v). glycogen phosphorylase b I glucose-6-P AMP A 315103-001 — MIDTERM 2 {A E f May 20, 2010 Name A / 7 Last, First (print clearly, please) 4. (18 pts). a. (8 pts). Starting with citrate in the mitochondria, how many ATPs are required to synthesize the fatty acid shown below? For full credit, you must explain the source(s) of ATP. Write your answer on the line provided. 315103-001 - MIDTERM 2 E May 20, 2010 Name E 8 Last, First (print clearly, please) b. (10 pts). Starting with fatty acid shown in below in the cytosol, how many net ATPs are produced from the oxidation of the fatty acid to C02? For full credit you must explain your answer, indicating the sources of ATP. Do not write out the pathways. WRITE YOUR ANSWER ON THE LINE PROVIDED. 3 i A f‘ O "i f! 5 C": HSCW/ !\0 w 5 i, S L .m ‘ ATP yield t 1 . \J 4/ Q I 2+ . M.w«...-—,... _ V‘Nr/ 5?; (a merge. (DA V. )2. er: :1, if C; 744-}: x I: f ‘6 Hr ' a: " r > V _, _ m my 5 Nl’rt‘JH ‘7‘: 'fi m-hflm’; [5‘ if” lfir‘mfll‘fi 1”? [’XFADPEZ 7\ 2 3 f3: \ 9. A 2:: I Lisé 19/9le "WM . .w Fore“ Doom: Bovo ; I 318103-001 — MIDTERM 2 lL May 20, 2010 Name Z 9 Last, First (print clearly, please) 5. (7 pts). The following oxidation-reduction reactions are catalyzed by one or more enzymes acting in sequence. Which complexes and/or enzymes are required to catalyze the following overall reactions? Write the name of the complex(es) and/or the name of the enzyme (s) in the space provided. Do not guess- 0.5 points will be deducted for wrong answers. List in any order on the lines provided. You don’t have to put an answer on all lines. a. succinate + 1/2 02 —> fumarate + H20. H H H Cvmglm 1T. , M , , , b. NADH + H+ + 2 cytochrome c (Fe+3) —> NAD+ + 2 cytochrome c (F e+2) + 2H“ H H M_,M‘IH, , , c. CoQH2 (reduced) + 1/2 02 —> CoQ (oxidized) + H20 -H +\ ‘ ' (olewIU, , , BISlO3-001 — MIDTERM 2 May 20, 2010 Name 10 Last, First (print clearly, please) 6. (9 pts). You are raising turkeys. For their dietary source of carbon, you feed one group of turkeys 14C-labeled succinate, and a second group of turkeys you feed 14C-labeled palmitic acid (018:0). After 24 hours, you take a liver sample out of one turkey from each group. Both livers contain 14C-labeled glycogen. After a month of these same diets, the turkeys are weighed. The turkeys in the group fed 14C-labeled palmitic acid (C 18:0) do not weigh as much as the turkeys in the group fed 14C-labeled succinate. a) (6 pts). Explain qualitatively why the two groups of turkeys grew differently® ' I} it "H ~ ft m, n'k’: .' ‘1» b 7"" r; a. 5 V C2 {At/4 filer-f "1C OOWW‘). if L u? I L1,: i 3 r :5 ill) g? l‘ l I ' ":1 am? I?" (x n: ; fi’xryvn ‘15, v,” f L). .{y r “ . it.» i E I A: ) ( w a» Mr A. 1; V1 Li”? i”?- “15%” b). (3 pts). Would the result be different if a 14C-labeled Cl7 fatty acid (017:0) were fed to the turkeys instead of the l4C-labeled palmitic acid (C 18:0)? Yes or No and briefly explain why... » :1 . n»; _ q “u u 3&3)ij " i . 5 -~’ .i v . r r ; - ’ wt” l .-~’. g ’ 4 ‘ we ‘ . y ' r - g n. .a_ l .' g r ; "an ‘5 f ,r "1 °\, Gd :1 C“ “q x?! {JFK ~ :4 V L '_ V. -.,. a” as“) ix, "an, I it, 3 .. z; (3., t - ‘2“... 1 WV!- .-*.;:.<, ‘v . _ .. w. ‘ V r“ >91 s ; .‘k Ca m2 s» y (I ‘: a ‘3‘“ J 315103-001 — MIDTERM 2 K E V May 20, 2010 Name Last, First (print clearly, please) 11 7. (10 pts). (a). ( 5 pts). Under standard conditions, what is the minimum AEO' required for the synthesis of 1 mole of ATP from ADP and inorganic phosphate by ATP synthase? For full credit, you must show your work. Assume 100% efiiciency. Md #0 W032. ’305 16%» {19 R0111 mama Basalme +1 X :mc 113%; l 12"” (b). (5 pts). What is the maximum AEO' available in humans from the electron transport chain for the synthesis of ATP? For full credit, you must show your work. 'P/Uot’l TAbvé.“ +1 ‘ \ 0/on +H “ANA-D +26+3H+ +610 i" 501+9H’l41g—5HLO +.‘51(9"J‘I [/36 MN. B18103-001 — MIDTERM 2 K May 20, 2010 Name 12 Last, First (print clearly, please) 8. (18 pts). Multiple Choice. Circle the best answer. There is only one best answer per question. Each question is worth 2 pts. a. Which of the following are properties of ATP synthase? 1. The F1 subunit is attached to the integral membrane protein F0 2. The F0 subunit is an integral membrane subcomplex 3. The F0 subunit forms a H+ pore through the membrane, which is otherwise impermeable to H+s 4. The F1 is the site of ATP synthesis 5. The F0 subunits form a proton gradient-dependent rotor l of the above 7. None of the above. b. The complete reduction of one molecule of oxygen gas by the Electron Transport Chain requires how many electrons? c. Three reactions of B-oxidation of saturated fatty acids are analogous to which sequence of metabolic reactions discussed previously? ® succinate —> fumarate —> malate —> oxaloacetate 2. isocitrate —> a-ketoglutarate -* succinate —> fumarate 3. oxaloacetate —-> citrate —> isocitrate -—> a-ketoglutarate 4. a-ketoglutarate —> succinyl CoA —> succinate ——> fumarate 5. pyruvate —-> oxaloacetate —> phosphoenolpyruvate —-> 2-PGA BISIO3-001 — MIDTERM 2 May 20, 2010 Name 14. 13 Last, First (print clearly, please) (1. Adenylate cyclase 1. catalyses the synthesis of ATP 2. catalyses the synthesis of AMP @catalyses the synthesis of cAMP 4. is localized to the inner mitochondrial membrane. 5. catalyses the activation of fatty acids for beta oxidation. e. Non-lactating mammary tissue metabolize about 10% of the total glucose used via the oxidative pentose phosphate pathway. In lactating mammary tissue, the fraction of the total glucose used by the pentose phosphate pathway increases to 40%. The biochemical reason for this increase is as follows: 1. The oxidative pentose phosphate pathway produces ATP, which is required for increase biosynthetic activity during milk production. 2. The oxidative pentose phosphate pathway produces galactose, which is needed for increased milk sugar (lactose) biosynthesis. 3 A direct product of the oxidative pentose phosphate pathway is glutathione, which is important for keeping cellular proteins in the reduced and active state. KG) The oxidative pentose phosphate pathway produces NADPH, which is need for -\/ increased fatty acid synthesis. 5. The extremely high level of ATP in lactating mammary tissue inhibits PFK-l, which decreases glycolysis, thus increasing flux through the oxidative pentose phosphate pathway. BISlO3-001 — MIDTERM 2 K May 20, 2010 Name 14 Last, First (print clearly, please) The next three questions below refer to the metabolic pathway shown below. E1 E2 E3 B4 A <—>B—>CHD—>E E1-E4 are enzymes A through E are metabolites The statements below refer to this pathway. Circle the correct answer(s). f. Feedback inhibition refers to: Q metabolite E binding to enzyme E2. 2 metabolite A binding to enzyme E2. 3 metabolite D binding to enzyme E4. 4. metabolite B binding to enzyme E3. 5 metabolite A binding to enzyme E4. g. Feed-forward activation refers to: 1. metabolite E binding to enzyme E2. 2. metabolite B binding to enzyme E1. ® metabolite A binding to enzyme E2. 4. metabolite C binding to enzyme E2. 5. metabolite C binding to enzyme E1. h. The most likely KPIE (Key Physiologically Irreversible Enzyme) of this pathway is: 1. E1 (5) E2 3. E3 4. E4 5. none of the above BI5103-001 — MIDTERM 2 ’L E May 20, 2010 Name 15 Last, First (print clearly, please) i. Plants, but not mammals, carry out the series of reactions called the glyoxylate cycle. This pathway is important to plants because it enables them to: 1. obtain glyoxylate for synthesis of amino acids 2. oxidize acetyl CoA to C02 without consuming NAD+ 3. form acetyl-CoA fi'om malate @‘r carry out the net synthesis of glucose from acetyl-CoA 5. catabolize glyoxylate to C02 ...
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This note was uploaded on 10/22/2010 for the course BIS 102 taught by Professor Hilt during the Spring '08 term at UC Davis.

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BIS103 MT2 S10 KEY - 318103-001 — MIDTERM 2 Name k 3 Last First(print clearly please 1(10 pts Fill in the blanks by writing the answer on the

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