FST123 Final Practice S99 Smith

FST123 Final Practice S99 Smith - N ame Sample FST 123...

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Name:___ Sample_______ FST 123 Final Examination Spring, 1999 Values of some useful constants: the Boltzman constant, k B = 1.38 x 10 -16 erg/degree•molecule the gas law constant, R = 1.98 cal/mole•degree Planck's constant, h = 6.627 x 10 -27 erg•s 1. Give a brief description of the principles behind the following techniques or methods: (16 points) a. size exclusion chromatographic separation (gel filtration) of proteins b. precipitation of proteins by addition of a miscible organic solvent c. measurement of protein concentration using the method of Bradford. d. a photoaffinity label 2. List five ways in which protein can differ from one another that can be used as means to separate and/or purify them and give one technique that makes use of each of the properties you list. (20 points)
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2 of 9 3. Classify the enzymes that catalyze the following reactions according to the EC nomenclature numbering system by assigning the first digit (18 points). a. __________________________ CH 2 OP O O O HC HC OH OH CH HO HC OH C OH CH 2 O O O HC HC OH OH HO O CH C CH 2 OH Glucose-6-Phosphate Fructose-6-Phosphate b.__________________________ CH 2 O O O HC OH H O C CH 2 O O O HC HC OH OH HO O CH C CH 2 O O O O C CH 2 O O O CH 2 OH + c. __________________________ + H O 2 C O HO CO CH 2 P O O O C O HO CH O CH 2 OH P O O O d.__________________________ CH 3 C O O O O ADP ATP CH 3 C O O + +
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3 of 9 4. Sugar (i.e., sucrose) is hydrolyzed by the enzyme invertase: O OH H H O H H OH H H OH CH 2 OH OH CH 2 OH CH 2 OH OH H H CH 2 OH CH 2 OH HO O H H OH H OH OH CH 2 OH OH Sucrose Glucose Fructose H 2 O Invertase + It can be assumed that the hydrolysis of simple disaccharides proceeds by attack of water on the anomeric carbon atom of the monosaccharide on the non- reducing end of the disaccharide, to cleave the acetal bond. But, sucrose is a
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This note was uploaded on 10/22/2010 for the course FST 123 taught by Professor Smith during the Spring '09 term at UC Davis.

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FST123 Final Practice S99 Smith - N ame Sample FST 123...

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