FST123 MT1 S99 Smith

FST123 MT1 S99 Smith - Midterm Examination FST 123 Spring...

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Midterm Examination FST 123 Spring ’99 Name 1) The following diagrams show three common types of secondary structure. For each of them, identify the kind of secondary structure shown and indicate two reasons that the structure is more stable than other possible structures. (9 points) a. Name b. Reason 1 c. Reason 2 d. Name e. Reason 1 f. Reason 2 g. Name h. Reason 1 i. Reason 2
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2 2) A protein has 12 glutamate and 7 aspartate residues plus 14 lysine residues. Ignoring the C- and N-termini, and assuming that the carboxyl groups have a pKa of 4.0 and the lysine sidechain has a pKa of 10, calculate the pI of the protein. (10 points) 3) The following table lists the properties of several proteins. (16 points) Protein Mr (monomer) Subunits pI total charge at pH 8.0 A 14,200 1 9.5 +12 B 13,900 1 5.6 -7 C 67,000 1 5.2 -12 D 67,000 2 5.2 -24 a. Would you expect that the following pairs of proteins could be separated by SDS gel electrophoresis? C & D yes no probably probably not A & C yes no probably probably not b. Would you expect that the following pairs of proteins could be separated by native gel
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FST123 MT1 S99 Smith - Midterm Examination FST 123 Spring...

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