October 18 - Lecture 14:Genes Genomics and Chromosomes...

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October 18, 2010 Lecture 14 :Genes, Genomics and Chromosomes – Eukaryotic Chromosomes Three functional elements are required for replication and stable inheritance o Telomere o Centromere Attachment site of sister chromatids o Multiple Origins of Replication (In most eukaryotic cells) Replication events Homologous chromosomes pair up, sister chromatids are bonded together at the centromere o Centromere is also the site of attachment for the mitotic spindle Experiments with Yeast Start with a yeast cell with a mutation in a gene for leucine biosynthesis o Will not grow unless the media is supplemented with leucine o Lack the gene necessary for leucine biosynthesis Plasmid with wild-type leucine biosynthetic gene is introduced into the cells lacking this gene o Still nothing grows on media lacking leucine We are looking for elements that allow such cells to replicate on media lacking leucine ARS confer the capacity to replicate in a yeast cell o But the colonies that form are small and irregular in shape While the ARS was capable of conferring the capacity of leucine, it wasn’t allowing the cells to segregate properly into daughter cells at mitosis o Where mitosis initiates A centromere allowed for replication of cells into daughter cells containing introduced plasmid
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October 18 - Lecture 14:Genes Genomics and Chromosomes...

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