September 29

September 29 - September 29, 2010 From Last Class Double...

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September 29, 2010 From Last Class Double strand DNA break repair by homologous recombination o Less change of errors 1. Two homologous chromosomes are end to end. The ends are digested by exonucleases, leaving 3’ single-stranded ends 2. RecA- (bacteria, organelles) or Rad51- (nuclear chromosomal) mediated strand invasion 3. 3’end of invading strand is extended by DNA polymerase until the displaced single strand base pairs with the other 3’ single strand generated initially. Migration of the branch occurs such that the extended broken 3’ end binds with the intact 4. The other available 3’ end of the other strand of the broken strand is extended by DNA polymerase 5. Ends are ligated 6. Cleave phosophodiester bonds and ligate alternative ends 7. This leaves two stretches, each of two strands, each strand containing a sequence that was originally from a strand from the other strand (See Pictures on Slide) Molecular Biology Techniques To be covered o DNA cloning o Polymerase Chain Reaction (PCR)
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This note was uploaded on 10/23/2010 for the course BIOL BIOL 200 taught by Professor Bureau during the Fall '09 term at McGill.

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September 29 - September 29, 2010 From Last Class Double...

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