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BICH 410 - Biochemistry 410 Homework H4 Due on 1(2 pts The...

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Biochemistry 410 Homework H4 Due on 10/25/2010 1. (2 pts) The energy of activation of an uncatalyzed reaction is 60 kJ/mol. The energy of activation is reduced to 25 kJ/mol for the enzyme catalyzed reaction. Please calculate the rate constant of the catalyzed and uncatalyzed reactions. How many folds of rate enhancement is produced by the enzyme? 2. (2 pts) Draw a V o versus [S] plot and a double reciprocal plot using the experimental data below and estimate the V max and K m of the enzyme catalyzed reaction from both of the plots. Compare your results from the two methods and explain why they are slightly different. [S] ( μ mol/L) V o (mM/min) 0.05 0.22 0.1 0.34 0.2 0.53 0.4 0.74 0.8 0.91 1.6 1.04 3.2 1.16 3. (2 pts) The kinetics of an enzyme is measured as a function of substrate concentration in the presence and absence of 2 mM inhibitor. The data from the experiments are shown below: [S] ( μ M) V o ( μ M/min ) V o ( μ M/min ) with 2 mM inhibitor 3 10.4 4.1 5 14.5 6.4 10 22.5 11.3 30 33.8 22.6 90 40.5 33.8 a. Please draw a set of V o versus [S] and corresponding double reciprocal plots to
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