Bio524_10_Lectures_9_and_10_corn_smut_fungus

Bio524_10_Lectures_9_and_10_corn_smut_fungus - genes is...

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How biotrophic fungi colonize plants: a tale of effectors Regine Kahmann Max Planck Institute for Terrestrial Microbiology Dept. Organismic Interactions
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Smuts and their symptoms loose smut on wheat bermuda grass smut barley sm anther smut sugarcane smut head smut
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Corn smut: Ustilago maydis Huitlacoche: ‘Mexican truffle’
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The life cycle of Ustilago maydis
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The early biotrophic phase PIN1-YFP tagged maize plasma membrane
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Late stages of biotrophic development
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The secretome of U. maydis (based on the full genome sequence) Prediction of 554 secreted proteins, of these only 168 are enzymes 178 genes code for completely novel putative secreted effectors A significant number of the novel effector genes are arranged in clusters The expression of most cluster
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Unformatted text preview: genes is strongly upregulated during the biotrophic phase Effectors selected for functional analysis are: Predicted to be secreted (N-terminal signal peptide) Lack similarity to proteins in databases Are transcriptionally upregulated during the biotrophic phase Effector gene clusters SG200 SG200/pep1 pep1 Effector mutants fail to penetrate and elicit a defense response pep1 mutants penetrate the plant cuticle, but become arrested at this stage SG200/pep1/mCHERRY Pin1-YFP (maize plasma membrane) (Doehlemann et al. PLoS Pathogens , 2009) pep1 mutants induce papilla formation and autofluorescence SG200/pep1 Pep-GFP, RFP cytoplasmatic, 3dpi Pep1 localization Go to Science paper...
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This note was uploaded on 10/24/2010 for the course BIOL 524 taught by Professor Dangl during the Fall '10 term at UNC.

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Bio524_10_Lectures_9_and_10_corn_smut_fungus - genes is...

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