Unformatted text preview: 2. Hypothesis: The growth of the contaminant was inhibited by a drop in pH Four tubes: first one contains only enterococcus, second and third ones contain both enterococcus and the contaminant, and the forth contains only the contaminant, should be prepared. Record the initial pH. Then, add a buffer to one of the tubes that contain both the enterococcus and the contaminant, in order to keep the pH from dropping. Incubate the tubes. Then streak cultures from the four broths onto the 4 sectors of vitamin free minimal medium agar plate and observe growth. The controls of the experiment include the broths with only the enterococcus, only contaminant and the broth with the buffer solution. If our hypothesis were correct then, the non-buffer condition broth would not have growth after incubation, and growth should be observed under the buffer condition....
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- Microbiology, #, buffer solution, Yueyue Shen Biomi