lar report - growth curve - graph, when the optical density...

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Yueyue Shen Lab Section- Tu/Th 10:10 Instructor- Carole Rehkugler Biomi 2911 Growth curve lab report 1). Results: We measured optical density values of E. coli cells in 5 tubes of varying temperature (22 °C, 30 °C,37 °C, 45 °C and 50 °C) every 10-15 minutes for a total of about 90 minutes. A line of best fit was drawn, which best represented the data points. The measured absorbance values were then recorded in Table 1. Table 1 - doubling time and growth rate of E. coli in five tubes with varying temperature Tubes of E. coli Doubling time (minutes) Growth rate (generation / hr) 22°C ~ 36 min = ~ 0.6 hr 1.67gen/hr 30°C ~ 30 min = ~ 0.5 hr 2gen/hr 37°C ~23 min = ~ 0.38 hr 2.6gen/hr 45°C ~25 min = ~0.416 hr 2.4 gen/hr 50°C Temperature ( o C) of ~
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Yueyue Shen Lab Section- Tu/Th 10:10 Instructor- Carole Rehkugler Biomi 2911 Growth curve lab report 50°C ~ 59 min = ~ 1hr 2. en/hr Sample calculation: In the tube with temperature 22 °C, the optical density at 12 minutes was 0.1. According to the
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Unformatted text preview: graph, when the optical density is 0.2 (2 x 0.1), the corresponding time is 48 minutes. Therefore the doubling time for the culture in temperature 22 C is approximately 36 minutes. The growth rate is the reciprocal of the doubling time. 36 minutes is 0.6 (3/5) of an hour and the reciprocal is 5/3, which is approximately 1.67gen/hr. 2). The optimal temperature, according to the table is somewhere around 37C. 3). My hypothesis: the optimal temperature for E. coli growth would be around 37C, because E. coli were found in the humans, and the average temperature of the human body was around 37C and. The least growth would be observed around 50C. Based on the data collected in class, the optimal temperature for E. coli growth is 37C, and the least growth would be observed around 50C. Thus, the data supported the hypothesis....
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This note was uploaded on 10/24/2010 for the course BIOMI 2911 at Cornell University (Engineering School).

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lar report - growth curve - graph, when the optical density...

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