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lar report - growth curve

# lar report - growth curve - graph when the optical density...

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Yueyue Shen Lab Section- Tu/Th 10:10 Instructor- Carole Rehkugler Biomi 2911 Growth curve lab report 1). Results: We measured optical density values of E. coli cells in 5 tubes of varying temperature (22 °C, 30 °C,37 °C, 45 °C and 50 °C) every 10-15 minutes for a total of about 90 minutes. A line of best fit was drawn, which best represented the data points. The measured absorbance values were then recorded in Table 1. Table 1 - doubling time and growth rate of E. coli in five tubes with varying temperature Tubes of E. coli Doubling time (minutes) Growth rate (generation / hr) 22°C ~ 36 min = ~ 0.6 hr 1.67gen/hr 30°C ~ 30 min = ~ 0.5 hr 2gen/hr 37°C ~23 min = ~ 0.38 hr 2.6gen/hr 45°C ~25 min = ~0.416 hr 2.4 gen/hr 50°C Temperature ( o C) of ~

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Yueyue Shen Lab Section- Tu/Th 10:10 Instructor- Carole Rehkugler Biomi 2911 Growth curve lab report 50°C ~ 59 min = ~ 1hr 2. en/hr Sample calculation: In the tube with temperature 22 °C, the optical density at 12 minutes was 0.1. According to the
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Unformatted text preview: graph, when the optical density is 0.2 (2 x 0.1), the corresponding time is 48 minutes. Therefore the doubling time for the culture in temperature 22 °C is approximately 36 minutes. The growth rate is the reciprocal of the doubling time. 36 minutes is 0.6 (3/5) of an hour and the reciprocal is 5/3, which is approximately 1.67gen/hr. 2). The optimal temperature, according to the table is somewhere around 37°C. 3). My hypothesis: the optimal temperature for E. coli growth would be around 37°C, because E. coli were found in the humans, and the average temperature of the human body was around 37°C and. The least growth would be observed around 50°C. Based on the data collected in class, the optimal temperature for E. coli growth is 37°C, and the least growth would be observed around 50°C. Thus, the data supported the hypothesis....
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lar report - growth curve - graph when the optical density...

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