BISC220_Exam1_9AM_answers

BISC220_Exam1_9AM_answers - BISC 220: Exam #1 Multiple...

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Unformatted text preview: BISC 220: Exam #1 Multiple Choice Answers Version #1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 A B B D C A C A D A C B C B C B C B C E D A D D D Blank D D C B C Version #2 C 1 B 2 A 3 D 4 C 5 A 6 A 7 C 8 B 9 C 10 B 11 C 12 B 13 D 14 B 15 B 16 D 17 C 18 C 19 E 20 D 21 A 22 D 23 D 24 C 25 Blank 26 D 27 D 28 C 29 B 30 A 31 Version #3 B 1 D 2 C 3 C 4 A 5 E 6 C 7 D 8 A 9 B 10 E 11 E 12 B 13 A 14 A 15 A 16 B 17 D 18 C 19 D 20 E 21 C 22 E 23 D 24 A 25 A 26 B 27 B 28 D 29 B 30 A 31 Version #4 E 1 B 2 C 3 C 4 A 5 A 6 C 7 E 8 D 9 A 10 B 11 E 12 D 13 B 14 A 15 A 16 B 17 D 18 C 19 D 20 A 21 D 22 A 23 A 24 B 25 B 26 B 27 C 28 A 29 D 30 E 31 Lab Portion Bisc 220 Midterm 1 - 9AM (20 pts total) Multiple Choice. 1.You ran the Benedict Test for the sucrose solution and obtained a clear blue color. After the experiment, you left the sucrose solution at room temperature overnight instead of putting it back into the refrigerator. The next day you ran the Benedict Test again using the same sucrose solution. To your surprise you obtained a red-orange solution instead. What likely happened to the sucrose solution that resulted in the false positive result? (2 pts) A. B. C. D. E. It has been degraded to glucose and fructose. It has been degraded to glucose It has been degraded to glucose and lactose. The sucrose formed a polysaccharide. It formed a coil that reacts with the Benedict reagent. 2. In the iodine test for starch, the iodine (2 pts) A. B. C. D. E. binds to the outside of the amylose giving off a blue color. fails to bind amylose and remains colorless. becomes entrapped by the helix of the amylose giving off a blue color. binds to β-strands of proteins within the starch and gives off a red-brown color. reduces the starch giving off a blue color. Fill in the blanks (5 pts). 3. The substrate for peroxidase is hydrogen peroxide (H2O2) and the products are water (H2O) and oxygen (O2). When one of the products is coupled with guaiacol, the reaction changes from colorless to a brown color. Short Answers 4. Why was alcohol added to the DNA solution before you spool it with a glass rod? Explain in 2-3 sentences. (5 pts) DNA is soluble in water. When alcohol is added, DNA becomes insoluble in solution. Therefore it can be spooled. 5. Describe in 2-3 sentences the principle of gel filtration chromatography. Be sure to focus on the matrix and the properties of the biomolecules. (6 pts) Dextran is a larger molecule than the orange dye. Larger molecules does not fit into the small spaces of the matrix and flows through it. Smaller molecules spends more time going through the small spaces in the matrix therefore elutes later. ...
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