Lecture 11- pharmacology and NMDA+LTP

Lecture 11- - Today we will focus on the postsynaptic side of the synapse and consider the various types of receptors that neurons possess and what

Info iconThis preview shows pages 1–14. Sign up to view the full content.

View Full Document Right Arrow Icon
Today we will focus on the postsynaptic side of the synapse, and consider the various types of receptors that neurons possess and what actions those receptors have on the postsynaptic cell
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 transporter
Background image of page 2
3 fusion Tethered vesicles clathrin dynamin
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4
Background image of page 4
5 Shibire mutant flies have a mutation in their dynamin that only affects dynamin function at higher temperatures. This prevents vesicle recycling and thus deplete their vesicles at the neuromuscular junction when heated above 30 degrees Celsius
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
6 After Koenig and Ikeda (1989). J. Neuroscience 9, 3844. @29 C A Close Look at Shibire Terminals @22 C
Background image of page 6
7 Movie of Shiberi mutants
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Three criteria for identification of a substance as a neurotransmitter 1) Nerve stimulation must cause the release of the substance and the release must be shown to come from the nerve. 2) It must be shown that the effects of nerve stimulation are mimicked by applying the suspected neurotransmitter directly to the postsynaptic cell. 3) Responses to nerve evoked and extrinsic application must display the same response to various drugs, i.e., the responses must have the same pharmacology .
Background image of page 8
Discovery of Acetylcholine as a Neurotransmitter fulfills the first criterion The classic experiement performed By Otto Lowei in 1921
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
There are 2 major types of acetycholine (ACh) receptors 1) Nicotinic ACh receptors-excitatory 2) Muscarinic ACh receptors-inhibitory Each type is distinguished on the basis of pharmacology (how the receptors respond to drugs) We turn first to Nicotinic ACh receptors at the neuromuscular junction
Background image of page 10
Contraction force Time (sec) ACh is inhibitory to the heart muscle but is excitatory to skeletal muscle Stimulate nerve and evoke AP in muscle
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Pipette filled With ACh Pass current and eject ACh STIMULATE NERVE RECORDINGS OF MEMBRANE POTENTIAL THROUGH PIPETTE GIVE PULSE OF ACH THRU PIPETTE Preparation bathed in low Ca++ to reduce transmitter release The effects of aceytlcholine released by nerve terminals is mimicked by iontophoretic application of acetylcholine thereby fulfilling the second criterion
Background image of page 12
Nicotinic ACh receptors are found at the neuromuscular junction Nicotine acts as a substitute for ACh (it is an agonist) while curare blocks these receptors (it is an antagonist) Pipette filled with nicotine solution Pass current and eject nicotine STIMULATE NERVE RECORDINGS OF MEMBRANE POTENTIAL THROUGH PIPETTE GIVE PULSE OF ACH THRU PIPETTE Preparation bathed in low Ca++ to reduce transmitter release Stimulate nerve Pulse on nicotine
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 14
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/25/2010 for the course BIO 365R taught by Professor Draper during the Spring '08 term at University of Texas at Austin.

Page1 / 52

Lecture 11- - Today we will focus on the postsynaptic side of the synapse and consider the various types of receptors that neurons possess and what

This preview shows document pages 1 - 14. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online