{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 11- pharmacology and NMDA+LTP

Lecture 11- pharmacology and NMDA+LTP - Today we will focus...

Info icon This preview shows pages 1–15. Sign up to view the full content.

View Full Document Right Arrow Icon
Today we will focus on the postsynaptic side of the synapse, and consider the various types of receptors that neurons possess and what actions those receptors have on the postsynaptic cell
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2 transporter
Image of page 2
3 fusion Tethered vesicles clathrin dynamin
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
4
Image of page 4
5 Shibire mutant flies have a mutation in their dynamin that only affects dynamin function at higher temperatures. This prevents vesicle recycling and thus deplete their vesicles at the neuromuscular junction when heated above 30 degrees Celsius
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
6 After Koenig and Ikeda (1989). J. Neuroscience 9, 3844. @29 C A Close Look at Shibire Terminals @22 C
Image of page 6
7 Movie of Shiberi mutants
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Three criteria for identification of a substance as a neurotransmitter 1) Nerve stimulation must cause the release of the substance and the release must be shown to come from the nerve. 2) It must be shown that the effects of nerve stimulation are mimicked by applying the suspected neurotransmitter directly to the postsynaptic cell. 3) Responses to nerve evoked and extrinsic application must display the same response to various drugs, i.e., the responses must have the same pharmacology .
Image of page 8
Discovery of Acetylcholine as a Neurotransmitter fulfills the first criterion The classic experiement performed By Otto Lowei in 1921
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
There are 2 major types of acetycholine (ACh) receptors 1) Nicotinic ACh receptors-excitatory 2) Muscarinic ACh receptors-inhibitory Each type is distinguished on the basis of pharmacology (how the receptors respond to drugs) We turn first to Nicotinic ACh receptors at the neuromuscular junction
Image of page 10
Contraction force Contraction force Contraction force Time (sec) ACh is inhibitory to the heart muscle but is excitatory to skeletal muscle Stimulate nerve and evoke AP in muscle
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Pipette filled With ACh Pass current and eject ACh STIMULATE NERVE RECORDINGS OF MEMBRANE POTENTIAL THROUGH PIPETTE GIVE PULSE OF ACH THRU PIPETTE Preparation bathed in low Ca++ to reduce transmitter release The effects of aceytlcholine released by nerve terminals is mimicked by iontophoretic application of acetylcholine thereby fulfilling the second criterion
Image of page 12
Nicotinic ACh receptors are found at the neuromuscular junction Nicotine acts as a substitute for ACh (it is an agonist) while curare blocks these receptors (it is an antagonist) Pipette filled with nicotine solution Pass current and eject nicotine STIMULATE NERVE RECORDINGS OF MEMBRANE POTENTIAL THROUGH PIPETTE GIVE PULSE OF ACH THRU PIPETTE Preparation bathed in low Ca++ to reduce transmitter release Stimulate nerve Pulse on nicotine
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Nicotinic ACh receptors are found at the neuromuscular junction
Image of page 14
Image of page 15
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern