CHEM 4030 Assignment #2

CHEM 4030 Assignment #2 - V0 Asparaginase 1 Asparaginase 2...

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Asparaginase 2 Asparaginase 1 V 0 [S] CHEM 4030: Assignment II Due Friday, October 29, 2010 Name (Please print CLEARLY) GRADING SHEET MAXIMUM SCORE SCORE EARNED 50 This cover page must be stapled to the assignment you turn in. It is the front page of your assignment
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1. Which one of the following statements is true of enzyme catalysts? A) They bind to substrates, but are never covalently attached to substrate or product. B) They increase the equilibrium constant for a reaction, thus favoring product formation. C) They increase the stability of the product of a desired reaction by allowing ionizations, resonance, and isomerizations not normally available to substrates. D) They lower the activation energy for the conversion of substrate to product. E) To be effective they must be present at the same concentration as their substrates. 2. The steady state assumption, as applied to enzyme kinetics, implies: A) K m = K s . B) the enzyme is regulated. C) the ES complex is formed and broken down at equivalent rates. D) the K m is equivalent to the cellular substrate concentration. E) the maximum velocity occurs when the enzyme is saturated. 3. An enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the K m for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 μ mol. If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how long would it take for the same amount (12 μ mol) of product to be formed? A) 1.5 min B) 13.5 min C) 27 min D) 3 min E) 6 min 4. Which of these statements about enzyme-catalyzed reactions is false ? A)At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is proportional to the enzyme concentration. B)If enough substrate is added, the normal V max of a reaction can be attained even in the presence of a competitive inhibitor. C)The rate of a reaction decreases steadily with time as substrate is depleted. D)The activation energy for the catalyzed reaction is the same as for the uncatalyzed reaction, but the equilibrium constant is more favorable in the enzyme-catalyzed reaction. E)The Michaelis-Menten constant K m equals the [S] at which V = 1/2 V max . 5. To calculate the turnover number of an enzyme, you need to know: A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] >> K m . C) the initial velocity of the catalyzed reaction at low [S]. D) the K m for the substrate. E) both A and B. 6. What are the units for the Michaelis constant K M ? A) B) Time, in seconds or minutes C) Moles/liter
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D) Moles/sec E) Moles/liter-sec F) Per second 7. Assume that the reaction velocity for an enzyme reaction is 15 μM/min when [S] = K M . Calculate
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This note was uploaded on 10/26/2010 for the course BIO 3031 taught by Professor Horgen during the Spring '10 term at Hawaii Pacific.

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CHEM 4030 Assignment #2 - V0 Asparaginase 1 Asparaginase 2...

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