L17 - Biophysical Chemistry Chemistry 24a Winter Term...

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Biophysical Chemistry Chemistry 24a Winter Term 2009-10 Instructor: Sunney I. Chan Lecture 17 March 3, 2010 Magnetic Resonance II. Nuclear Magnetic Resonance NMR I. Background and Basic Concepts
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Introductory to NMR Spectroscopy References I. General readings: 1. Kurt Wüthrich (2003) “NMR studies of structure and function of biological macromolecules” J. Biomolecular NMR , 27: 13–39 (Nobel Lecture) 2. Schnell et al (2004) “Structure, Dynamics, and Catalytic Function of Dihydrofolate Reductase” Ann. Rev. Biophys. Mol. Biol. 33, 119 3. Boehr et al (2006) “The dynamic energy landscape of dihydrofolate reductase catalysis.” Science. 313 (5793):1638-42. II. Advanced Readings: 1. Henzler-Wildman1 & Kern (2007) Dynamic personalities of proteins” Nature 450 :964-72. 2. Boehr and Wright (2006) “An NMR perspective on enzyme dynamics.” Chem Rev. 106(8):3055-79 3. Tzakos et al (2006) “NMR Techniques for Very Large Proteins and RNAs in Solution” Ann. Rev. Biophys. Biomol. Struct. 35, 319. III. Text books: 1. Wüthrich, K. “NMR of Proteins and Nucleic Acids”, Wiley, 1986. 2. Cavanagh, J. et al., “Protein NMR Spectroscopy-Principles and Practice”, Academic Press, 1996. NMR History 1924 Pauli proposed the presence of nuclear magnetic moment to explain the hyperfine structure in atomic spectral lines 1930 - Nuclear magnetic moment was detected using Stern-Gerlach experiment by Estermann - Rabi first detected nuclear magnetic resonance signal. 1946 - Purcell et al reported nuclear resonance absorption in paraffin wax. - Bloch et al at Stanford found nuclear induction signal in water. 1950 Theoretical development of NMR 1960 - Chemical shift phenomenon was discovered - Fourier Transform technique was introduced by Ernst and Anderson 1970 - Solid state NMR revitalized by Waugh & associates at MIT - Superconducting high-field magnet become available and NMR was applied to macromolecules - Feasibility of magnetic resonance imaging (MRI) was demonstrated - Multi-dimensional NMR was introduced. 1980s Protein structure was first determined by Kurt Wuthrich at ETH. 1990s Macromolecular structure determination by NMR become routine 2000 Applications to more complex systems, with focus on dynamics.
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Felix Bloch 1905-1983 Physics, 1952 Edward M. Purcell 1912-1997 Physics, 1952 Kurt Wuthrich 1938- Chemistry 2002 Richard R. Ernst 1933- Chemistry, 1992 Isador I. Rabi 1898-1988 Physics, 1944 Paul C. Lauterbur 1929-2007 Medicine 2003 Mike Mansfield 1933 Medicine 2003 NMR spectroscopy Nuclear magnetic resonance (NMR) is spectroscopy associated with the interaction between a nuclear magnetic dipole in an atom or molecule with an externally applied magnetic field B . Absorption or emission of rf is induced by the interaction of the nuclear magnetic dipole with the H rf field of the light. Vector quantities are underlined.
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Two ways to describe the interaction: Energy of interaction: - μ m ·B a pplied for each spin Torque: μ m x B applied = dJ /dt [ x cross product] E for spin ½ nuclei, e.g.
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This note was uploaded on 10/27/2010 for the course BI 110 taught by Professor Richards,j during the Winter '08 term at Caltech.

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L17 - Biophysical Chemistry Chemistry 24a Winter Term...

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