PreLab_SDS-PAGE - 3. What is the purpose of the APS and the...

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Names: Marsha Taylor, Jason Jeffries, and Robert Serpas Prelab Assignment SDS-PAGE Background Preparation 1. Either provide the MW of the yeast cyt. c (if you isolated this) or an estimated MW for the deer heart cyt c. What animal did you estimate the cyt. c from? The molecular weight of bovine is 11833 Daltons, so 11833 grams/mole 2. How will you prepare 500mL of 1X running buffer from a 20X stock solution? Provide numbers. To prepare this you would use: C1V1=C2V2 C1 = 20X, V1 (unknown), C2 =1X, V2 = (500mL) Plug in the values and solve for the unknown (mL) The unknown Volume (mL) is = (1X)(500mL) / (20X) = 25mL Add 25mL of the 20X stock solution to enough dH2O to attain a final volume of 500mL.
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Unformatted text preview: 3. What is the purpose of the APS and the TEMED? The TEMED reaction is initiated by the APS. The TEMED catalyzes the polymerization of the gel. 4. Why is glycerol, a substance more viscous and dense than water, part of the sample prep mix? Glycerol is added to make the sample fall to the bottom of the well. Without glycerol the sample could mix with the buffer and flow out of the top reservoir. 5. Distinguish between SDS-PAGE and PAGE. SPS-PAGE complexes are denatured and PAGE complexes are not. This allows the PAGE complexes to retain their natural folds. 6. In SDS-PAGE, do the proteins migrate toward the anode or to the cathode? The proteins migrate to the anode....
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