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Unformatted text preview: 3. What is the purpose of the APS and the TEMED? The TEMED reaction is initiated by the APS. The TEMED catalyzes the polymerization of the gel. 4. Why is glycerol, a substance more viscous and dense than water, part of the sample prep mix? Glycerol is added to make the sample fall to the bottom of the well. Without glycerol the sample could mix with the buffer and flow out of the top reservoir. 5. Distinguish between SDS-PAGE and PAGE. SPS-PAGE complexes are denatured and PAGE complexes are not. This allows the PAGE complexes to retain their natural folds. 6. In SDS-PAGE, do the proteins migrate toward the anode or to the cathode? The proteins migrate to the anode....
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- Spring '10