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Lab Manual- Appendix II for Microscale

Lab Manual- Appendix II for Microscale - Appendix II...

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8/26/2010 A pp e n di x I I : M I CR OS CA L E E X T R A C TI O N S e e a l s o P av i a: T ec hn i qu e 12 . 5 & 12 . 6 The extraction process consists of two parts (i) mixing of the two immiscible solutions, and (ii) separation of the two layers after the mixing process. Organic layer more dense than aqueous layer (Fig. 1) Mixing. (a) The aqueous solution is added to the vial containing the unknown mixture and dichloromethane. (b) The vial is capped and shaken to thoroughly mix the two phases. The vial is carefully vented by loosening the cap to release any pressure that may develop. (c) The vial is allowed to stand on a level surface to allow the phases to separate. A sharp boundary should be evident. Separation (a) The pipette bulb is squeezed to force air from the pipette. (b) The pipette is then inserted into the vial until close to the bottom. Be sure to hold the pipette in a vertical position. Fig 1: Pasteur pipette separation of two immiscible phases, with denser layer containing the product.
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