8 - 4 5 The gene products (Env, RT, gag) are supplied by...

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1 Picornaviruses Single stranded (+) RNA genome, ~8kb. Can be translated as an mRNA directly.
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2 Influenza Viruses There are two molecules on the viral envelope H (Hemagglutinin) : 15 serotypes N (Neuramindase) : 10 serotypes Each serotype has many subtypes with minor mutations. Lentivirus/Retrovirus
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3 Retrovirus Transfer vector: 1. 5’LTR (promoter) 2. 3’LTR (poly A signal) 3. Packaging signal 4. Strong promoter (CMV) 5. Drug marker 6. No env, pol nor gag genes Retroviral transfer vector
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Unformatted text preview: 4 5 The gene products (Env, RT, gag) are supplied by the packaging cell lines. The genes are separated from each other in the packaging cell lines’ genomes so that they won’t be packaged into the recombinant virus. Packaging Cell Lines Retrovirus http://www.clontech.com/clontech/expression/retro/retro2.shtml Adenovirus http://www.clontech.com/clontech/expression/adeno/index.shtml 6 Adenovirus d.s. DNA genome ~35 kb Adenovirus transfer vector 7 8...
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This note was uploaded on 10/28/2010 for the course SCI biol3214 taught by Professor Lam during the Spring '10 term at CUHK.

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8 - 4 5 The gene products (Env, RT, gag) are supplied by...

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