pm2_1d - BICD | 00 ' WINTER 200? MIDTERM3 l. Unicorns have...

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Unformatted text preview: BICD | 00 ' WINTER 200? MIDTERM3 l. Unicorns have typical diploid eukaryntic genetics. You are studying,1 three genes in unicerns whose recessive mutant alleles cause the pitentitvpes of miniature silver. and striped. ‘r'nu cross unieoms from a pure-breeding silver striped strain to unicorns l‘mm a pure breeding miniature strait‘tao tibtairt Fl Ulticnl‘tts. You test-cross these Fl unicorns to a pure-breeding silver striped miniature strain and analyze 500 F2 progeny. You obtain the following data: Parents: silver striped X miniature F2 progeny: # mutant pltenotypdst inferred gamete alleles _ miniature silver striged 3 silver .4 5. 1 34 miniature silver W St 1. 186 SliVLI’ stt’tped + S’ 3+ 49 miniature silver striped N. a; $1 —_%—__—_#—___ l 86 miniature m t a 5 mlmature strtped - W. _. if 14 striped 4 + at |——-—————————.—.—_—_—— 34 Wlld type ‘ t + .t w- a. State the genotype of the FI unicoms . 1' . _. I 1e. ;. "I . Li'ii‘*l4.4_ 2 . 1t . 1,. 51‘ ' Viv-Tun" si‘ '- [v it'."TJ[.-# : i.-.r‘.ri'.1‘\'"'3- we. __ ‘-.-ti,i-11 PL e 1/ hi Fill in the gamete genotypes int eta meta ove (the gamete produced by the Fl fly 3. wt. .- ark to produce the indicated F3111}t 'phenotypie class) it"? "3|" “Lot/a BICDIOU WINTER 2007’ MIDTERM2 c. Derive the most accurate map you can 01' the order of these genes and the distances between them. State the amount of interference. if any. To receive credit you must SHOW YOUR WORK HERE: “1"” 54".)“ = 7* 2" * 5+“! ,3 fl 2 '12. H; 53:- 54: if 54hr; I“ “A +1“ mgr”; s-‘va «Mm = 24 r H‘H “5+3”, '33. :. 2s: ’A FF eat: 9’" were“? 3 “WW3”- .13, m: 2: Eu ‘- 593 51h“, ‘fl'v-fdl- “wifikw‘L q “mu-u f3 m.“- I Dr nus) 2+5 7 I u( 2 1 —‘ 7 0‘8“ {-er 1‘6 Gllr'lflf‘plv‘ UL mm (or?) (o.aa)(a.m)[5») R" _ ’- oee 2 MW In he’ll-NINE ‘ ANSWER (draw your final map here) Interference = Page 3 of? BICDIUU WINTER 3007 MIDTERMZ 2. You cross a gal4 haploid yeast strain to a budl haploid yeast strain to obtain a diploid that is heterozygous for both genes: gal4 BU D+ x GAL+ budl gal4 BUD+ diploid GAL+ budt This yeast species produces unordered tetrads. For each of the following situatigns—diagoanuhe 4-chromatid stage of a meiosis producing a Non-Parental DitypelidthW' The diagram should show the location(s) of crossoveris] if any. an o early indicate which chromatids segregate together in order to explain the genotypes of the 4 spores. For full credit you must show the minimum number of crossovers that could explain the tetrad type. If there is more than one way to obtain the specified tetrad type using the same number of crossover events, Show only one example. a) if gal4 and budl genes are linked: 1+ H “64" ‘Hdnl'n I."r-V'{'I f ' r: 'L-' "' (1.“ _' .-_ ‘ 3‘3 \ (‘th q I r! 1 -J nfir l-l I J I .-_i (:Jwi + [‘q i? f” i + 17‘» 0" b) if gal4 and budl are unlinked. and budt is tightly linked to its oentromere. 1.:- h i--. H” (ach I . - J I - | J gs“: . \. 'I f "1 he I {l X. .i E M ': “H .. _I 1 -t1 I ' I Flat ‘ Page 4 of i“ BICD ] [)0 WINTER 2007 MTDTERMZ 3. In yeast Saooharomyces cerevisiae you cross two haploids: sr'n4 biui' x lei/2. In the resulting diploid. all three genes are heterozygous. From the following unordered tetrads, determine the linkage relationships between these three genes. including linkage to any oentromeres that can be determined. You do not have to determine if there is interference. FOR FULL CREDIT YOU MUST SHOW YOUR WORK. Tetrad genotyges number oi tetrads Equ '. 55"” 5"”. * x + * I" 2‘ sind biu+ iew- 69 sin4 him is»? _ Sim blu? law Fl 1 an” tofu! 1* srn+ biut iev2 1 f I“ 1 Sim? bio? few 82 51ft“ 3:“? :9“? 5.». ~ Wu. 1 I M90 sm+ u+ ev+ - - p u) NFD sin+ biu+ tel/2 It 2P9 FD a m :- Hn‘h‘karp I an4 bill-P {EV-P- 311 E PD @ ;. 5;“ r all“ {a (n. Lniur sin4 bio law in TT I + sin+ bio! few? I I aim bio? lam? 3"” ' [4" ' I 1" 1% , (I) Pb >) MP0 sin4 blur iev+ 299 1 It: a L: M. sin4 blur iev+ --- ‘1'” " sin+ biu+ iev2 _ _-‘. -_- I} i sin+ biu+iev2 16" 9-5 Tr ' “PP a "‘3 (3" F52) - :L—(r—J—‘J fi'riz ‘" : ___._.._.--"” J . Io-l'fll RF Tm, m 1“ Elm. ANSWER {draw your final map here) Bin? th‘l-Vbnfil H_S [Av L Page 5 of 7 BlCDIUO WINTER 2007 MIDTERM2 4. In bacteriophage. suppose you have three genes, a b and c, and you have been told that their genetic map is: c: a b 4 13 To verify this map you use co-infection to cross two strains of phage. one of which is a+ b+ c~, and the other is a- b- 0+. List all the possible phenotypic classes of progeny from this cross. and state how many of each you predict if you analyze 5000 progeny. Assume there is no interference. SHOW YOUR WORK. AND PUT YOUR FINAL ANSWERS IN THE TABLEBELovy. “ml: {1+ U 4 ft I; L- C" 9+ L‘ f’ a“ I"- F! I r" id [9+ ‘ ‘1 fl, ‘fl'i'b u“. Tt . + A 59.xo-E7Lire3fi-W‘L‘J: 9m I”) c n }’Drr{n+lll I - 3 of (re:- ® f" a. 19 Thus e) r- a 1“ } tutu. ripe): {=-='~r.)(-°-@}{‘5°*‘-’) I a4 F (in S. j OD (+ b '5 ‘ I?! if “(R :9 (o auflEmJ - 2" 3 W _ 3'? SF h-CL # PHEDICTED L? c_ of L—}( (a w) (o.i3)(5aag) -2.(!- : (937 pubs - 6 Ft: 1" .g‘F {UflL l; w ‘ Page 6 of T BICDIOU WINTER 200? MIDTERM2 5. In fruit flies the gene pr (mutant phenotype = purple eyes) is located on the X chromosome. Use the symbol X" to indicate an X chromosome with the wild type allele. X"r to indicate an X chromosome with the mutant allele, and Y to indicate a Y chromosome. a) Suggest the genotypes of a female and male fly you could cross, such that the parents have different phenotypes (purple eyes vs. wild-type red eyes). and when segregation is normal. all the female progeny will have their father‘s phenotype. and all the male progeny will have their mother’s phenotype (“cries cross inheritance“). ?f vr . . ‘V notype: X 7\ Father genotype: K j Ph otype: owQVE. Q‘JK‘Q‘S Phenotype: Lad-AER '\‘\{€E YEA Qqfl Mother b) Draw a Punnett Square to show how the cries-cross inheritance arises in your proposed cross: (5‘ «\x gquKZQOC ' c) In the cross abo e. suppose you RA ELY (< 1/1000) e unexpected case of a male that looks like his father (patroclinus exception). State what the gametes must be from each parent that combined to create the patroclinus exception, and state how such gametes could arise: non ._ cl igluull'ti U ’\ Q C) 76 d) What about a rare female that looks liket a mother (matroclinous { d exception)? State what the gametes must be from each parent that combined to create the matroclinus exc tion. . “50-m1_“““1 3%.. _\v \ imam¢1 China-n.1,“. X caucsm'i‘ QQER'LJU T W + r (are. x x x x Y A (otwa / Page 7 of 'Ir' ...
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This note was uploaded on 10/28/2010 for the course BICD 100 taught by Professor Nehring during the Fall '08 term at UCSD.

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pm2_1d - BICD | 00 ' WINTER 200? MIDTERM3 l. Unicorns have...

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