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Unformatted text preview: and SAMR0918 was predicted to code for a protein that regulates congocidine biosynthesis. The rest of the 19 genes direct either the biosynthesis of congocidine precursor or the assembly of the molecule. However, later the authors found that this gene cluster is not involved in the biosynthesis of the three congocidine precursors but NRPS was observed to catalyze the amide bonds formation between the precursors and the 4 genes that code for NRPS has been identified. One significant proposal of the paper is the assembly of congosidine. Activated pyrrol precursors are first transferred to cgc18_A PCP and cgc19, condensation then takes place to form bipyrrole molecule. The addition of other 2 activated precursors is catalyzed by cgc2 and 16, followed by N-methylation by cgc15. In sum, the paper is very straight forward and easy to understand. The authors postulates are fairly convincing due to detailed experimental procedures and results....
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- Spring '07