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reading 1 - Intro 1 The rising interest in enzyme is partly...

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Intro 1. The rising interest in enzyme is partly due to site-directed mutagenesis method. 2. Mutagenesis method serves as a useful tool for understanding structure-function relations by mutating the amino acid sequence of a given enzyme. 3. Enzyme engineering: site-directed mutagenesis techniques, hybrid enzymes, semi- synthetic enzymes, and totally synthetic enzyme-mimetic compounds, catalytically active antibodies 4. Pauling: enzyme is a “flexible molecular template, designed by evolution to be precisely complementary to the reactants in their activated transition-state geometry, as distinct from ground-state geometry.” 5. Enzyme strongly binds to the transition state, increasing its concentration and accelerating the reaction. A Brief Chronology 1. Transition-state stabilization principle: slow evolution 2. Modern theory: (Haldane) a. “enzyme-substrate complex requires additional energy of activation” to react b. Fischer’s lock-and-key model does not work. Suggested substrate strain/distortion 3. Contemporary theory (transition-state theory): (Eyring) Transition-state complex is in equilibrium with the reactants. However, transition-state stabilization theory was not wide spread and only played a minor role in enzymological thinking until 1969. 4.
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This note was uploaded on 10/28/2010 for the course CHEM 157 taught by Professor burkart during the Spring '07 term at UCSD.

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reading 1 - Intro 1 The rising interest in enzyme is partly...

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