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reading 6 final

reading 6 final - through gene duplication and random...

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In the research article “Chimeras of Two Isoprenoid Synthases Catalyze All Four Coupling Reactions,” the authors suggest that four coupling enzymes involved in isoprenoid compounds synthesis could have evolved from a common ancestor. The authors have found 75% identity and 96% similarity exist in sagebrush FPPase, a chain-elongation enzyme, and sagebrush CPPase, an cyclopropanation enzyme. By replacing segments of the FPPase with corresponding sequences from CPPase, the authors were able to generate chimeric proteins, which are used to catalyze the four fundamental isoprenoid-coupling reactions. The four reactions give similar mechanisms and absolute stereochemsitries of the chiral products, which lead to the proposal that the four coupling enzymes are evolved from a common ancestor
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Unformatted text preview: through gene duplication and random mutagenesis of the duplicate genes. In sum, this article is fairly convincing. The authors represented their studies very well by both explaining the reactions in details and by providing adequate figures to show the mechanisms. However, the article should include the crystal structures of the enzymes to further demonstrate the similarity. Considering the similar mechanisms of the reaction, the absolute stereochemistry of the products, and the conserved isoprenoid fold in avian FPPase, sterol biosynthesis and several terpenoid cyclases, the likelihood the four coupling enzymes being evolved from a same common ancestor is high....
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