Lec8 - Proteins & polypeptides Properties of actual...

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Proteins & polypeptides
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Properties of actual proteins There are 20 n number possible polypeptides of n residues long with 20 different AA Only a very small fraction seen in nature Minimum chain length is 40 residues for a polypeptide to fold into a protein Not all 20 AA appear in similar frequencies in proteins (Trp, Met, Cys are AAs in proteins) Protein sizes are also limited to avoid error in translation Some proteins contain single polypeptides whereas others contain multiple polypeptide chains
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Inclusion Body
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Separation techniques Characteristic Procedure Solubility Salting out Charge Ion exchange chromatography Electrophoresis Polarity Hydrophobic interaction chromatography Size Gel filtration Electrophoresis Ultracentrifugation Binding specificity Affinity chromatography
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Chromatography Discovered in 1903 to separate pigments present in plants
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Enzyme-Liked Immunosorbent Assay
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Fractionation by salting out
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Charged molecules bind to opposite charged groups on the column
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This note was uploaded on 10/28/2010 for the course CHEM 114A taught by Professor Viadhiu during the Fall '08 term at UCSD.

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Lec8 - Proteins & polypeptides Properties of actual...

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