Lec19 - Membranes are Fluid Fluid mosaic model: proteins...

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Fluid mosaic model: proteins float freely in a sea of lipids This model may only be partially true, not all parts of a membrane are accessible to all proteins Membranes are Fluid
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Diffusion of labeled proteins 1. Human and mouse cells with differently labeled protein are allowed to fuse by virus
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2. Human and mouse proteins are segregated immediately after fusion 3. After 40 min human and mouse proteins are fully intermixed
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Cell fusion experiments
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FRAP: Fluorescence Recovery After Photo Bleaching 1. Bleaching of fluorophore a small area by laser 2. Intact fluorophores diffuse into the bleached area
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Erythrocyte Membrane Easy to isolate Isolate from blood by centrifugation Lyse erythrocytes by hypotonic lysis Cell contents leak out Carries out only few metabolic processes Essentially is a bag of hemoglobin No organelles
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Erythrocyte Membrane Skeleton
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Biconcave disc; ensures rapid O2 diffusion Shape of erythrocytes is determined by a skeleton of proteins underlying the membrane
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Erythrocyte Membrane Proteins 6-7 peripheral membrane proteins 7-8 integral membrane proteins SDS-PAGE analysis of Erythrocyte membrane proteins Spectrin Actin Band 4.1
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Structures of Spectrin and Ankyrin Proteins
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75% of erythrocyte ghost membrane is composed of spectrin Two chains (a chain=280 kDa; b chain=246 kDa) form a heterodimer; heterodimer further associates to form
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This note was uploaded on 10/28/2010 for the course CHEM 114A taught by Professor Viadhiu during the Fall '08 term at UCSD.

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Lec19 - Membranes are Fluid Fluid mosaic model: proteins...

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