ProblemSet2 - Chemistry 114A(Problem Set 2 Fall 2007 1 A...

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Chemistry 114A (Problem Set 2): Fall 2007 1. A 4.0kb linear DNA fragment is cut with the following restriction enzymes or combination of enzymes, producing the DNA fragment listed. Endocuclease(s) DNA fragments (in kb) EcoRI 2.8 and 1.2 PstI 1.8, 1.3 and 0.9 EcoRI and PstI 1.8, 1.0, 0.9, and 0.3 Produce a linear restriction map consistent with the data shown in the table above. 2. Give the 2 major structural differences between the DNA strand and the RNA strand. 3. In the double helical structure of DNA described by James Watson and Francis Crick, the nitrogenous base guanine pairs with the base ____________ by forming ___________ hydrogen bonds. A) adenine;three B) cytosine;three C) adenine;two D) cytosine;two E) adenine;four 4. Which of the following DNA strands is complementary to 5'-GAACTGATC-3' F) 5'-CTAGTCAAG-3' G) 5'-CTTGACTAG-3' H) 5'-GATCAGTTC-3' I) 5'-GAACTGATC-3' J) 5'-GAACUGAUC-3' 5. Which of the following was NOT a discovery that directly lead to Watson and Crick’s determination of the DNA double helical structure? K) Rosalind Franklin’s X-ray diffraction experiments on DNA fibers L) Oswald Avery’s discovery that DNA is the material of heredity. M) Erwin Chargaff’s observation of the equimolar amounts of nucleotide purines and pyrimidines from DNA preparations N) Jerry Donohue’s understanding of keto-enol tautomerism in DNA bases O) Cyrus Levinthal’s paradox of protein folding 2) The double helical structure of DNA determined by Watson and Crick in 1953
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This note was uploaded on 10/28/2010 for the course CHEM 114A taught by Professor Viadhiu during the Fall '08 term at UCSD.

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ProblemSet2 - Chemistry 114A(Problem Set 2 Fall 2007 1 A...

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