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ProblemSet4 - Chemistry 114A, 2007 Problem Set #4 1. Amino...

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Chemistry 114A, 2007 Problem Set #4 1. Amino acid analysis of a peptide revealed only seven different amino acids where concentrations of some amino acids are higher than the others: The following facts of a peptide were noted: A) Carboxypeptidase A treatment yielded the amino acid threonine B) One cycle of Edman analysis released the PTH-adduct of arginine C) Treatment with staphylococcal protease (Glu-C) yielded a tripeptide and hexapeptide D) Chymotrypsin treatment of the peptide released a pentapeptide and tetrapeptide. The peptapeptide when treated with cyanogens bromide two dipeptides and tyrosine is released. E) Treatment of tetrapeptide generated from chymotrypsin treatment with β -mercaptoethanol yielded two identical dipeptides What is the amino acid sequence of this peptide? 2. A biochemist is attempting to separate a dimeric RNA-binding protein (protein X) from a mixture of 7 other proteins (proteins 1 to 7). These proteins have the following properties: pI Size (MW) Binds to RNA Protein 1 10.0 82,000 no Protein 2 3.8 21,500 no Protein 3 7.9 23,000 yes Protein 4 9.8 22,000 yes Protein 5 5.5 43,000 no Protein 6 6.0 110,000 no Protein 7 8.5 37,000 yes Protein x 10.1 65,000 yes Explain what type of separation techniques can be used to separate protein X from the mixture. 3. Examine the following sequences, and suggest whether they prefer a-helix, b- sheet or neither (random coil or loop). Give reasons. Arg-Thr-His-Val-Tyr-Phe-Ala-Ile
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ProblemSet4 - Chemistry 114A, 2007 Problem Set #4 1. Amino...

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