lec9 - Lecture 9 Pyruvate Dehydrogenase and Uses of...

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Unformatted text preview: Lecture 9 Pyruvate Dehydrogenase and Uses of Intermediates of the Citric Acid Cycle 1 What happens to pyruvate? Complete oxidation (aerobic) Muscles (anaerobic) Yeast (anaerobic) Pyruvate Dehydrogenase Pyruvate Decarboxylase Lactate Dehydrogenase 2 Compartmentalization • Pyruvate transported to mitochondrion (pyruvate-H + symporter) Cytosol Mitochondrion 3 Pyruvate to Acetyl-CoA NADH CO 2 NADH CO 2 GTP FADH 2 NADH Pyruvate dehydrogenase 4 Pyruvate dehydrogenase complex • Pyruvate dehydrogenase (E 1 ) • Dihydrolipoyl transacetylase (E 2 ) • Dihydrolipoly dehydrogenase (E 3 ) • ~4600 kDa • Diameter ~300 Å • Core 24 E 2 in a cube • 24 E 1 and 12 E 3 around core • Similar to α-ketoglutarate dehydrogenase 5 Pyruvate Dehydrogenase Complex: E 1 • Decarboxylation of pyruvate • Uses thiamine pyrophosphate (TPP) as cofactor — vitamin B 1 6 Thiamine Pyrophosphate (B 1 ) Useful in decarboxylation reactions of α-keto acids Forms carbanion (C- ) 7 • TPP required for stabilization of carbanion (C- ) • TPP also in pyruvate decarboxylase (ethanol fermentation) Thiamine Pyrophosphate (B 1 ) 8...
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This note was uploaded on 10/30/2010 for the course CHEM 114B taught by Professor Wang during the Winter '09 term at UCSD.

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lec9 - Lecture 9 Pyruvate Dehydrogenase and Uses of...

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