lec23 - Lecture 23 DNA synthesis and Nucleoside Breakdown 0...

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0 Lecture 23 DNA synthesis and Nucleoside Breakdown
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1 Synthesis of DNA A,G,C, U A,G,C, T Ribonucleoside diphosphate deoxyribonucleoside diphosphate Ribonucleotide reductase
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2 Ribonucleotide Reductase (Class I) (ADP,
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3 Tyrosine Free Radical Binuclear Fe(III) O 2 - ion Tyrosine free radical
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4 Ribonucleotide Reductase Free radical Cys redox Disulfide (needs to reduced later)
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5 Reduction of Ribonucleotide Reductase
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6 dNTP dNDP + ATP dNTP + ADP Nucleoside diphosphate kinase no preference between ribose and deoxyribose between bases
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7 Regulation of Ribonucleotide Reductase
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8 Regulation of Ribonucleotide Reductase: balanced pool for DNA sysnthesis
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9 Purines Pyrimidines
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10 dUTP + H 2 O dUMP + PP i dUTP diphosphohydrolase (dUTPase) Tight pack of Tyr prevents 2’-OH Hydrogen bonds: specificity for U
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11 Thymidylate (dTMP) is synthesized from dUMP by thymidylate synthase DHFR: important drug target
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12 inhibitor of Thymidylate Synthase (kill cancer cells)
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13 Inhibition of DHFR THF
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14 nucleotide nucleoside From the diet Nucleoside degraded to base + ribose by nucleosidase Nucleoside degraded to base + ribose- 1-P by reaction with Pi by nucleoside phosphorylase
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15 Adenosine deaminase and AMP deaminase Mammalian Purine Nucleoside Phosphorylase (PNP) does not degrade A or dA Deficiency — SCID
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16 Adenosine deaminase and AMP deaminase SCID (severe combined immunodeficiency disease)
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This note was uploaded on 10/30/2010 for the course CHEM 114B taught by Professor Wang during the Winter '09 term at UCSD.

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lec23 - Lecture 23 DNA synthesis and Nucleoside Breakdown 0...

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