Discussion section 0505

Discussion section 0505 - Discussion se ction Transcription...

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Discussion section Transcription 05/09/08
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Central Dogma DNA --------> RNA ------> Protein transcription translation Gene mRNA Translation at ribosome Protein
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(RNA) n+1 + PP i 2P i (RNA) n + NTP H 2 O The Reaction Initiation Elongation Termination DNA + RNA Three phases
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RNA transcription in E.coli
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MgCl 2+ “hand” shape
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Gene Transcription +1 gene downstream upstream
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+1 Regulation of transcription in E.coli: Operons - transcription units that can be independently regulated monocistronic RNA or polycistronic RNA +1
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Promoter Sequences in E.coli for Initiation sense strand binding by RNA holoenzyme: partial DNAse1 digestion evolutionary conservation: consensus sequences functional test: mutation affects gene transcription rate How do we know these sequences are important?
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Promoters: locations where proteins are bound DNAse 1 footprinting : to identify protein-DNA interaction sites
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DNAse1 Footprinting
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-9 to +2
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holoenzyme has high affinity for promoter DNA but low affinity for other DNA core enzyme has high affinity for all DNA σ is important for promoter recognition: holoenzyme binds only loosely to non-promoter regions allows for rapid search for promoter regions different σ factors modulate the gene specificity of RNA polymerase RNA polymerase: he role of σ http://www.science.siu.edu/microbiology/micro302/transcription.html
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This note was uploaded on 10/30/2010 for the course CHEM 114C taught by Professor Alexanderhoffmann during the Spring '08 term at UCSD.

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Discussion section 0505 - Discussion se ction Transcription...

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