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lecture5 - Replication in Eukaryotes 501 very processive...

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501 Replication in Eukaryotes
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502 δ very processive (with PCNA, the clamp) 3’-5’ exonuclease but no primase no exonuclease only 100nt processive associates tightly with primase - lagging strand pol? ε very processive 3’-5’ exonuclease for UV damage -leading strand pol?
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503
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504 PCNA ß subunit
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505 85-355 fragment of DNA polymerase ß Klenow fragment: 2 domains - polymerase - 3’-5’ exonuclease
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506 => multiple origins: every 3-300kb (replicons) slower speed: 50 vs. 1000nt /s more DNA: 3 x 10 9 vs. 4.6 x 10 6
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507 Primer removal requires two enzymes
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508 DNA sequences required for eukaryotic chromosome replication Identified with a genetic complementation system
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509 An element required for replication: ARS autosomal replicating sequence (1) ARS (replication origin) ~100 bp
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510 An element for segregation: The Centromere (2) CEN (centromere)
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511 Figure 20-29 Stages of mitosis and cytokinesis: The role of the Centromere
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512 Centromeres: required for chromosome segregation during mitosis Lodish Fig. 20-33 MBOC Fig. 4-49 H3= CENP-A in humans (Centromere binds to the kinetichore)
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513 Eukaryotic chromosomes Scanning electron micrograph images of chromosomes
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514 Linear DNA requires telomere sequences at the ends for stable replication
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515 Functional elements of a chromosome required for replication and stable inheritance
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516 Telomeres TTAGGG repeats (humans and other vertebrates) at end of chromosomes A few hundred (yeast) to a few thousand (vertebrates) base pairs. The 3’-end of the telomere extends 12-16 bp beyond the 5’ end of the complementary strand. Proteins bind to telomeres to protect the ends of chromosomes.
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