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dissociation ikb nfkb association ikk ikb

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Unformatted text preview: FkB!! Dissociation IkB-NFkB!! Association IKK-IkB!! Dissociation IKK-IkB!! Export NFkB!!! Import NFkB!!! Export IkB-NFkB!!! Import IkB-NFkB!!! Export IkB!!! Import IkB!!! Degradation IkB!!! Degradation IkB-NFkB!! Degradation IKK-IkB!! Degrad. IKK-NFkB-IkB! IkB Protein synthesis biochem. b1 b2 b3 b4 b5 b6 b7 b8 b9 b10 b11 b12 b13 b14 b15 b16 b17 b18 b19 b20 b21 b22 b23 b24 b25 groupings genetic pharm. IKK 45,47,49 αβε 7,9,11 16,18,20 1,3,5 IKK αβε 2,4,6 17,19,21 51,52,53 LMB LMB LMB MG132 MG132 MG132 MG132 CHX CHX CHX 22,24,26 23,25,27 8,10,12 54,55,56 αβε αβε 46,48,50 63,64,65 41,42,43 34,35,36 66,67,68 AAAAAAA 38,39,40 31,32,33 cytoplasm nucleus 44 37 60,61,62 αβε 28,29,30 13,14,15 αβε A IkB RNA degradation 57,58,59 α β ε 69 71 72 70 73 IkB RNA synthesis NF-kB responsive RNA synthesis NFκB target genes ikba-/ikbb-/ikbe-/ikba-/ikbe-/- TSA TSA TSA PDTC PDTC 1807 A mutant deficient in stimulus-specific temporal control WT IKK KA TNF LPS 0 10 15 30 45 60 90 120 0 10 15 30 45 60 90 120 min NFκBtemp computational simulation IKK IKK NF-κB NF-κB NF-κBn (nM) 100 100 less difference in the temporal profile 0 0 30 60 90 120 min 0 0 30 60 90 120 EMSA TNF LPS 0 15 30 45 60 75 90 105 120 0 15 30 45 60 75 90 105 120 min 1808 Stimulus-specific NFκB temporal control is critical for stimulus-specific gene expression NFκBtemp LPS TNF LPS wt TNF RANTES LIF G-CSF MIP-2 IP-10 L32 0 1 248 01248 01 234 6 8 01 23 468 Time (hr) 1809 Temporal c...
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This note was uploaded on 10/30/2010 for the course CHEM 114C taught by Professor Alexanderhoffmann during the Spring '08 term at UCSD.

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