lecture18

Lecture18

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Unformatted text preview: Combinatorial Control IFNβ enhancer Drosophila development Temporal Control p53 NFκB 1801 p53-mdm2 oscillations Are these happening in normal cells? If so, are they functionally important? 1802 NFκB is activated by many stimuli cytokines e.g. TNF signaling module Iκ B NF- κ B cytoplasm nucleus pathogens e.g. LPS IKK NF- κ B ε β α NF- κ B feedback mechanisms generate interesting temporal control of NFκB Is temporal control important? 1803 1804 (a red fluorescent proteins fused to NFkB) RFP-NFκB Stimulus-specific temporal control of IKK and NF-κB TNF LPS A: 79 B: 36 C: 22 D: 60 E: 111 NF-κB TNF LPS 0 10 15 30 45 60 90 120 IKK wt F: 16 G: 43 H: 51 I: 44 J: 134 K: 73 early and transient delayed and sustained TNF LPS 0 15 30 45 60 75 90 105 120 different gene expression programs EMSA IKK KA 1805 Is temporal control of NF-κB important for stimulus-specific gene expression ? TNF LPS TNF LPS IKK IKK NFκBtemp mutant module NF-κB NF-κB ?? 1806 A mathematical model of NFκB activation to study temporal control Inflammatory Signals IKK Parameter # 1,3,5,8,10,12,13,14,15 16,18,20,23,25,27,28,29,30 2,4,5,7,9,11 17,19,21,22,24,26 37 44 31,32,33 38,39,40 34,35,36 41,42,43 54,55,56 46,48,50 45,47,49 51,52,53 63 64 65 66 67 68 71 72 73 69 70 Description Association IkB-N...
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This note was uploaded on 10/30/2010 for the course CHEM 114C taught by Professor Alexanderhoffmann during the Spring '08 term at UCSD.

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